A new PCR-based method for HIV detection

描述（由申请人提供）：拟议研究的目的是开发一种超灵敏的光化学扩增方法，用于提高基于聚合酶链反应（PCR）的人类免疫缺陷病毒（HIV）检测的性能。一项新技术可以提高灵敏度、准确度、动态范围、信噪比，节省昂贵的试剂并缩短分析时间。这种独特的方法以前从未被用于改善这些方法的性能。我们建议进一步改进和优化新技术，以产生适合研究和临床实践的常规技术。因此，我们的建议具有诊断和筛选相关的目标，并将新的工程原理应用于生物学和医学问题。初步分析表明，这种新方法具有巨大的商业和科学潜力。

DESCRIPTION (provided by applicant): The objective of the proposed research is to develop an ultrasensitive photochemical amplification method for improving the performance of polymerase-chain reaction (PCR)-based detection of human immunodeficiency virus (HIV). A new technology allows one to increase the sensitivity, accuracy, dynamic range, signal-to-noise ratio, save costly reagents and reduce time of analysis. This unique approach has never been used before for improvement of the performance of these methods. We propose to further improve and optimize the new technology, so as to generate routine technique suitable for research and clinical practice. Thus, our proposal has diagnosis- and screening -related goals and applies new engineering principles to problems in biology and medicine. Preliminary analysis has shown that the new method has an enormous commercial and scientific potential.