BIACORE T100: PROTEOMICS

这个子项目是利用由NIH/NCRR资助的共享仪器赠款提供的资源的许多研究子项目之一。子项目和调查员(PI)可能从另一个NIH来源获得了主要资金，因此可能会出现在其他CRISE条目中。列出的机构是用于拨款的，而不一定是用于调查人员的机构。描述(由申请人提供)：本建议书中要求的仪器Biacore T100是一种测量表面等离子体共振(SPR)的自动化设备。Biacore仪器使用微流控系统测量SPR，该系统提供了定量分析能力，以详细研究两个分子之间结合的特异性、特定分子的浓度、蛋白质动力学以及两个蛋白质之间的结合亲和力。实时定量数据的产生将帮助乔治城大学的研究人员进一步了解蛋白质、多肽和核酸相互作用的方式和原因。这些知识将用于促进基础和应用科学知识的发展，重点放在癌症生物学和新的癌症疗法上。Lombardi能够从NIH盈余中获得二手Biacore 1000，并在我们开始使用SPR实现研究目标时生成初步数据。在乔治城大学使用B1000使我们有机会获得仪器和SPR方面的经验和专业知识。其中三个主要用户，Toretsky、Wellstein和Roy博士，已经使用B1000为他们的项目生成关键数据。所有三名研究人员都需要SPR测量来推进他们的项目，现在正在准备使用这些新数据的手稿。所有被列为主要用户的调查人员都资助了需要增强战略规划研究能力的项目。琼斯博士提出了一项建议，其中包括一个需要SPR的目标。Pestell博士和Rui博士都有多个已建立的项目，这些项目将从SPR中受益匪浅。这些好处在下面的项目部分中概述。我们的科学需求已经超出了B1000的能力。这些需求包括增强的检测较小分子相互作用的能力、温度动力学和更快速的生产能力，以适应乔治敦隆巴迪癌症中心日益增长的兴趣和越来越多的用户。T100的这些增强能力将使研究多种生物过程的项目得以推进。

This subproject is one of many research subprojects utilizing the resources provided by a Shared Instrumentation Grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the grant, which is not necessarily the institution for the investigator. DESCRIPTION (provided by applicant): The instrument requested in this proposal, the Biacore T100, is an automated device to measure surface plasmon resonance (SPR). The Biacore instrument measures SPR using a microfluidic system that provides quantitative analysis capabilities to study, in detail, the specificity of the binding between two molecules, concentrations of specific molecules, protein kinetics, and binding affinity between two proteins. The generation of real-time quantitative data will help Georgetown University investigators further understand how and why proteins, peptides, and nucleic acids interact. This knowledge will be used to advance both basic and applied scientific knowledge with a significant focus on cancer biology and novel cancer therapies. Lombardi was able to obtain a used Biacore 1000 from NIH surplus and generate preliminary data as we begin to work towards achieving our research goals using SPR. Working with the B1000 at Georgetown University has given us an opportunity to gain experience and expertise with the instrument and SPR. Three of the major users, Drs. Toretsky, Wellstein, and Roy, have used the B1000 to generate key data for their projects. All three investigators required the SPR measurements to advance their projects and are now preparing manuscripts with this new data. All investigators listed as Major Users have funded projects that require enhanced SPR capabilities. Dr. Jones has a proposal and includes an aim that requires SPR. Drs. Pestell and Rui both have multiple established projects that will benefit considerably from SPR. These benefits are outlined in their project sections below. Our scientific needs have exceeded the capacity of the B1000. These needs include the enhanced ability of detecting smaller molecule interactions, temperature kinetics, and more rapid throughput to accommodate the growing interest and increased number of users at Georgetown's Lombardi Cancer Center. These enhanced capabilities of the T100 will allow projects investigating many types of biologic processes to advance.