ISLET CELL BIOLOGY CORE

胰岛细胞生物学核心：导演-F·马辛斯基 这个核心的目的是帮助目前正在学习或计划进行研究的调查人员 独立或合作研究胰岛细胞生物学的各个方面。为了 实现这一目标核心提供四项服务。首先，它将执行额外的下士 小鼠和大鼠胰腺完整分离灌流的表型研究 切碎的胰腺。其次，它将分离、培养和评估胰岛。 包括批量孵化、灌流、呼吸测量、CaJ++测量、 P-电位(ATP、ADP、AMP和PI)及其他代谢产物、激素含量和释放。第三，它 维持广泛且具有良好特性的转化胰岛细胞群，培养大量 这种细胞或通过将这种细胞嵌入琼脂糖珠来产生假性胰岛 新陈代谢和激素释放的研究。第四，也是最重要的，它提供了深入的 咨询和帮助制定战略如何以最佳方式使用核心服务或将 尝试修改现有技术以解决特定问题。胰岛小区资源允许 广泛应用劳动密集型和技术要求过高的方法 由普通的单个实验室进行维护。该中心为那些拥有 他们希望在自己的实验室中建立经批准的程序和技术。 通过大规模运营降低了成本。因此，核心起到了研究支持的作用 和教育单位，为具有不同兴趣的活跃的调查小组提供支持，以及 促进跨学科的小岛研究。

ISLET CELL BIOLOGY CORE: Director - F. Matschinsky It is the purpose of this core to assist investigators who currently study or have plans to study independently or collaboratively various aspects of pancreatic islet cell biology. In order to accomplish this goal the Core provides four services. First, it will perform extra corporal phenotyping of the endocrine pancreas of mouse and rat using the intact isolated perfused or minced perifused pancreas. Second, it will isolate, culture, and functionally assess pancreatic islets of rat and mouse including batch incubations, perifusions, respirometry, measurements of Caj++, the P-potential (ATP, ADP, AMP and Pi) and other metabolites, hormone contents and release. Third, it maintains a broad and well characterized stock of transformed islet cells, grows large batches of such cells or generates pseudo islets by embedding such cells into agarose beads for dynamic studies of metabolism and hormone release. Fourth and most important, it provides in depth consultation and helps develop strategies how to use the services of the core optimally or will attempt to modify available technologies to solve particular problems. The islet cell resource permits the broad application of approaches which are too labor intensive and technically too demanding to be maintained by the average single laboratory. The core offers training to those who have the resources and wish to establish approved procedures and technologies in their own laboratory. Costs are reduced through a large scale operation. The Core functions thus as a research support and educational unit for an active group of investigators with somewhat diverse interests, and fosters interdisciplinary islet research.