VIRAL LOCALIZATION BY IN SITU HYBRIDIZATION

该子项目是利用NIH/NCRR资助的中心赠款提供的资源的许多研究子项目之一。子项目和研究者（PI）可能从另一个NIH来源获得主要资金，因此可以在其他CRISP条目中表示。所列机构为中心，不一定是研究者所在机构。猴免疫缺陷病毒（SIV）和SIV/HIV嵌合病毒（SHIVs）感染亚洲猕猴是研究HIV感染和AIDS发病机制的重要动物模型。此外，该模型还提供了一个挑战系统，用于评估旨在预防人类感染或艾滋病的疫苗和抗逆转录病毒药物的功效。我们设计了几种分子探针，用于定位感染SIV和SHIV的各种分离株和克隆的猴组织中的病毒RNA。这些探针用于测量受感染猴子组织中受感染细胞的数量和分布。我们也有探针与其他几种病毒病原体的靶RNA或DNA序列杂交，包括SRV-1，CMV，SV 40，EBV，猴疱疹病毒和细小病毒。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The infection of Asian macaque monkeys with simian immunodeficiency virus (SIV) and SIV/HIV chimeric viruses (SHIVs) serves as an important animal model for studying the pathogenesis of HIV infection and AIDS. In addition, this model provides a challenge system for evaluating the efficacy of vaccines and antiretroviral agents designed to prevent infection or AIDS in humans. We have designed several molecular probes for localizing viral RNA in tissues of monkeys infected with various isolates and clones of SIV and SHIV. These probes are used to measure the quantity and distribution of infected cells in the tissues of infected monkeys. We also have probes that hybridize with target RNA or DNA sequences of several other viral pathogens, including SRV-1, CMV, SV40, EBV, Herpes saimiri, and parvovirus.