BASAL HEPATIC GLUCOSE AND INSULIN SENSITIVITY IN BABOONS

这个子项目是利用由NIH/NCRR资助的中心拨款提供的资源的许多研究子项目之一。子项目和调查员(PI)可能从另一个NIH来源获得了主要资金，因此可能会出现在其他CRISE条目中。列出的机构是针对中心的，而不一定是针对调查员的机构。这项研究的目的是通过实施正常血糖-高胰岛素钳夹手术来评估狒狒的葡萄糖代谢，以比较肥胖、瘦小和糖尿病动物之间的葡萄糖清除的差异。正常血糖-高胰岛素钳夹手术被认为是测量胰岛素敏感性的金标准技术，是人类受试者的常规操作，将使我们能够评估这些动物的胰岛素敏感性。在正常血糖-高胰岛素钳夹过程中，循环中的葡萄糖通过输注外源性葡萄糖来保持恒定，以响应胰岛素水平的上升。作为该项目的一部分，我们还收集了肌肉、脂肪和肝脏的活检标本，同时接受胰岛素注射，以检查组织在葡萄糖利用、胰岛素信号传递和基因表达模式方面的特定差异。我们的主要目标是验证该方案在胰岛素-葡萄糖轴的分子遗传学长期研究中的有效性。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The purpose of this study was to conduct assessments of glucose metabolism by performing the euglycemic-hyperinsulinemic clamp procedure in baboons in order to compare the difference in glucose clearance between obese, lean, and diabetic animals. The euglycemic- hyperinsulinemic clamp procedure is considered the ¿Gold Standard¿ technique to measure insulin sensitivity, and is routinely performed in human subjects, and will allow us to evaluate insulin sensitivity in these animals. During the euglycemic-hyperinsulinemic clamp procedure circulating glucose is held constant by infusion of exogenous glucose in response to rising insulin levels. As part of this project we have also collected muscle, fat and liver biopsies while under insulin administration to examine tissue-specific differences in glucose utilization, insulin signaling, and gene expression patterns. Our major objective is to validate this protocol for long term studies in molecular genetics of the insulin-glucose axis.