ADAPTING MICROTITERPLATE ASSAYS TO AN AUTOMATED PLATFORM

该子项目是利用NIH/NCRR资助的中心赠款提供的资源的许多研究子项目之一。子项目和研究者（PI）可能从另一个NIH来源获得主要资金，因此可以在其他CRISP条目中表示。所列机构为中心，不一定是研究者所在机构。目的：该项目的目标是简化和标准化内分泌核心的常见分析测定，以便为科学家和动物管理人员提供快速，更可靠和更经济的服务。选择传统上使用微量滴定板格式的测定以适应自动化平台ACS-180。以前，雌酮结合物、孕二醇-3-葡糖苷酸、促卵泡激素和猴绒毛膜促性腺激素的免疫测定法被改编。在过去的一年中，促甲状腺激素和非激素分析，如转铁蛋白和纤连蛋白也进行了调整。 本项目的结果是当前能够使用ACS-180自动分析仪执行所有指定测定。这将缩短周转时间，降低成本，提高可靠性，并为更广泛的用户群提供服务。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Objective: The objective of the project was to simplify and standardize common analytical assays from the Endocrine Core in order to provide a fast, more reliable and more economical service to scientists and animal managers. Assays that have traditionally used a microtitper plate format were selected for adaptation to an automated platform, the ACS-180. Previously the immunoassays for estrone conjugates, pregnandiol-3-glucurinide, follicle stimulating hormone and monkey chorionic goadotropin were adapted. In the past year, thyroid stimulating hormone and non-hormone analyses such as transferrin, and fibronectin were also adapted. The results of this project is the current ability to perform all of the designated assay using the ACS-180 autoanalyzer. The advent will shorten turn-around time, reduce costs, provide increased reliability and provide service to a broader user base.