GLUCONEOGENESIS & KREBS CYCLE FLUXES IN HUMANS

这个子项目是利用由NIH/NCRR资助的中心拨款提供的资源的许多研究子项目之一。子项目和调查员(PI)可能从另一个NIH来源获得了主要资金，因此可能会出现在其他CRISE条目中。列出的机构是针对中心的，而不一定是针对调查员的机构。2型糖尿病(T2 DM)发病机制中的一个基本问题是葡萄糖产生过多的机制。近年来，多项研究评估了隔夜禁食后糖异生作用(来自TCA循环中碳骨架和甘油的葡萄糖之和)与糖原分解作用的对比。该项目的目标是改进在核心1和2中开发的用于人类常规应用的技术和概念。除了继续使用常规的2H和13C核磁共振分析不同代谢条件下的血浆和尿液外，我们还启动了3T和7T的研究，以测量人类骨骼肌细胞内的脂肪和糖原含量。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. A basic question in the pathogenesis of type 2 diabetes (T2DM) is the mechanism of excessive glucose production. In recent years multiple studies have assessed gluconeogenesis (the sum of glucose originating from carbon skeletons in the TCA cycle and glycerol) versus glycogenolysis after an overnight fast. The goal of this project is to refine the technologies and concepts developed in Cores 1 and 2 for routine applications in humans. In addition to continuing our work with conventional 2H and 13C NMR for analysis of plasma and urine under various metabolic conditions, we have initiated studies at 3T and 7T for measurement of intramyocellular lipids and glycogen content in skeletal muscle of humans.