Practical Proteomics: A training course for the BBSRC community

从广义上讲，蛋白质组学是对大量蛋白质的鉴定和表征，目的是通过揭示蛋白质水平和活性的变化来了解生物系统。由于蛋白质组学是一个相对较新的研究领域，目前在所有专业水平的生物和生化科学的许多领域的研究人员对现代蛋白质组学技术的实践培训有很大的需求。蛋白质组学中使用的不同技术非常多样化，需要生物科学家在他们不熟悉的新的和具有挑战性的领域成为专家。例如，质谱学技术绝对是蛋白质组学研究的核心，但很少有生物科学家对此有任何经验。如果利用蛋白质组学为生物科学提供的研究优势要适当和充分地为英国科学所用，这些技术需要被英国的生物科学家更广泛和有效地使用。因此，他们需要接受使用质谱学等技术的培训，并学习如何将蛋白质组学技术应用于他们自己的研究领域。提供必要的蛋白质组学技术培训的最好方法是让人们有机会在实验室里练习和学习它们。有了这种实践经验，生物学家将能够回到他们自己的实验室，并开始在他们自己的研究计划中有效地应用蛋白质组学。我们将通过向生物科学研究人员提供一门课程来满足蛋白质组学培训的需求，该课程将向他们传授蛋白质组技术的基础知识，并让他们有机会在实验室中实践这些技术。这门实践性的实践课程将持续五天，并结合课堂讲课、演示和实验室练习。除了关于蛋白质组学理论和实践的课堂讲座，课程的参与者将有机会与在蛋白质组学方面有经验的科学家讨论适当的蛋白质组学实验的设计。这门课程将由技术专家授课，他们在自己的研究中经常使用这些技术。学生在课程中使用的设备和仪器是最先进的，也是目前可用于蛋白质组学研究的最好的。学生们将学习如何从头到尾进行蛋白质组学研究，甚至可以在课程中携带自己的生物样本进行分析。我们所说的学生是指任何对蛋白质组学感兴趣并至少拥有生物或化学科学学士学位的人，包括研究生、博士后研究人员以及研究和学术工作人员(讲师、读者和教授)。有了在课程中获得的知识和经验，生物科学家将能够开始在他们自己的研究中使用蛋白质组技术。

Proteomics, broadly speaking, is the identification and characterisation of large numbers of proteins, for the purpose of understanding biological systems by uncovering changes in protein levels and activites. Because proteomics is a relatively new field of study, there is currently a great demand amongst researchers in many areas of the biological and biochemical sciences at all professional levels for hands-on training in modern proteomic technologies. The different technologies used in proteomics are very diverse and require bioscientists to become expert in new and challenging areas with which they are not familiar. For example, the technique of mass spectrometry is absolutely central to proteomics research, but few biological scientists have any experience with it. If the research advantages that using proteomics offers to the biosciences are to be properly and fully available to British science, these techniques need to be used much more widely and effectively by biological scientists in the UK. They therefore need to be trained to use techniques like mass spectrometry and be taught how proteomics technologies can be applied to their own areas of research. The best way to provide the necessary training in proteomics technologies is to give people the opportunity to practice and learn them in the laboratory. With this kind of hands-on experience, biologists will be able to return to their own laboratories and begin to apply proteomics effectively in their own research programmes. We will meet the need for training in proteomics by providing a course to researchers in the biological sciences which will teach them the basics of proteomic technologies and give them the chance to practice the technologies themselves in the laboratory. This practical, hands-on course will be five days long, and combine class-room lectures, demonstrations, and laboratory exercises. As well as class-room lectures on the theory and practice of proteomics, participants in the course will have the opportunity to discuss the design of appropriate proteomics experiments with scientists who are experienced in proteomics. The course will be taught by experts in the technologies, who are experienced at using them routinely in their own research. The equipment and instruments that will be used by the students on the course is state-of-the-art and the best that is currently available for proteomic research. The students will learn how how to carry out a proteomic study from beginning to end, and will even be able to bring their own biological samples to analyze during the course. By students, we mean anyone interested in proteomics with at least a first degree in the biological or chemical sciences, including post-graduates, post-doctoral researchers, and research and academic staff (lecturers, readers, and professors). With the knowledge and experience gained in the course, biological scientists will be able to start using proteomic technologies in their own research.