ANALYSIS OF EXOSOMES FROM CISPLATIN RESISTANT AND SENSITIVE OVARIAN CANCER

这个子项目是利用由NIH/NCRR资助的中心拨款提供的资源的许多研究子项目之一。子项目和调查员(PI)可能从另一个NIH来源获得了主要资金，因此可能会出现在其他CRISE条目中。列出的机构是针对中心的，而不一定是针对调查员的机构。Howell博士的团队通过微阵列分析发现，独特的外体标记在顺铂(DDP)耐药细胞中过度表达。对纯化的外切体的生化分析表明，耐药细胞释放的外切体明显富含顺铂的外排转运体。顺铂耐药细胞的外切体也积累了更高水平的顺铂，这增加了它们可能有助于耐药细胞输出药物的可能性(耐药细胞存在累积缺陷)。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Dr. Howell's group has found by microarray analysis that unique exosomal markers are over-expressed in cisplatin (DDP) resistant cells. Biochemical analysis of purified exosomes showed that resistant cells release exosomes that are significantly enriched in putative efflux transporters of DDP. The exosomes from DDP resistant cells also accumulate higher levels of DDP, raising the possibility that they maight be instrumental in the export of the drug from resistant cells (resistant cells are accumulation defective.)