LUMINESCENT IMAGING: TARGETING ATP

本子项目是利用由NIH/NCRR资助的中心赠款提供的资源的众多研究子项目之一。子项目和研究者（PI）可能已经从另一个NIH来源获得了主要资金，因此可以在其他CRISP条目中表示。列出的机构是中心的，不一定是研究者的机构。发光（光子发射）报告器提供了与传统荧光报告器不同的细胞活动视角，后者需要激发频率才能反应。发光报告蛋白虽然多样性少得多，但依赖于两个分子之间的化学相互作用，而这种相互作用依赖于要测量的目标。在这里开发的情况下，我们希望通过光子发射荧光素/荧光素酶反应记录ATP的细胞活性。荧光素是膜渗透的，作为一种蛋白质，如果有合适的载体，它可以被靶向和表达。获取这些矢量，并在我们的光子计数/成像系统中进行测试（见SPID 0094）是这个子项目的目标

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Luminescent (photon emitting) reporters offer different perspectives on cell activity than the more conventional fluorescent reporters ¿ the latter requiring an excitation frequency to react. Luminescent reporters, although far less diverse, depend upon and chemical interaction between two molecules where the association is dependent on the target to be measured. In the case being developed here we wish to record the cellular activity of ATP through the photon emitting luciferin/luciferase reaction. Luciferin is membrane permeable and as luciferase is a protein it can targeted and expressed if a suitable vector is available. Acquisition of such vectors, and their testing in our photon counting/imaging systems (see SPID 0094) are the goals of this subprojec