DEVELOPING RAPID RESPONSE ION SELECTIVE ELECTRODES

该子项目是利用NIH/NCRR资助的中心赠款提供的资源的许多研究子项目之一。子项目和研究者（PI）可能从另一个NIH来源获得主要资金，因此可以在其他CRISP条目中表示。所列机构为中心机构，不一定为研究者机构。这是生物流研究中心核心目标之一的子提案。我们目前正在开发具有改进的响应时间（<1 ms）和信噪比的离子选择性电极（ISE），以便从全局细胞外离子梯度中提取有意义的单离子通道特征。这些特征包括平均开放时间、电导和通道密度。在收集细胞数据和测试上述假设之前，有必要确定扩散过程对流出或流入点有限距离处离子通量的影响。因此，我们已经着手对细胞边界层的3D环境进行建模，并证明伊势是否可以可靠地从细胞外空间内的离子通道簇中解析单一离子电流。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. This is a subproposal within one of the core aims of the Biocurrents Research Center. We are currently developing ion-selective electrodes (ISEs) with improved response time (<1ms) and signal-to-noise ratio in order to extract meaningful single ion channel characteristics from global extracellular ionic gradients. These characteristics include mean open time, conductance and channel density. Prior to collecting cellular data and testing the above hypothesis, it is necessary to determine the influence of diffusion processes on ion fluxes at a finite distance from the point of efflux or influx. Therefore, we have set out to model the 3D environment of the boundary layer of the cell and demonstrate whether the ISE can reliably resolve unitary ion currents from clusters of ion channels within the extracellular space.