IDENTIFICATION OF IKK SIGNALING TARGETS

该子项目是利用NIH/NCRR资助的中心赠款提供的资源的许多研究子项目之一。子项目和研究者（PI）可能从另一个NIH来源获得主要资金，因此可以在其他CRISP条目中表示。所列机构为中心，不一定是研究者所在机构。Verma实验室项目涉及使用2D凝胶电泳，免疫沉淀和质谱法寻找IKK复合物的细胞靶点。通过合作，我们已经确定了几个潜在的细胞靶点。我们现在正在获得更多的数据来支持我们的假设，即这些目标中的几个确实是IKK复合物的底物，使用质谱法来确定体内磷酸化的位点。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The Verma lab project involves finding cellular targets of the IKK complex using 2D-gel electrophoresis, immunoprecipitation, and mass spectrometry. In collaboration, we have identified several potential cellular targets. We are now in the process of obtaining additional data to support our hypothesis that several of those targets are indeed substrates of the IKK complex, using mass spectrometry to determine sites phosphorylated in vivo.