AUTOMATED DETECTION OF REGIONS OF INTEREST IN MEMBRANE TRANSPORTERS

该子项目是利用NIH/NCRR资助的中心赠款提供的资源的许多研究子项目之一。子项目和研究者（PI）可能从另一个NIH来源获得主要资金，因此可以在其他CRISP条目中表示。所列机构为中心，不一定是研究者所在机构。以前，NIH/GM资助的膜转运蛋白药物遗传学（PMT）研究项目将膜转运蛋白中的单核苷酸多态性（SNP）发现区域限制在外显子和这些外显子周围的短侧翼序列，导致很少利用其他基因组项目提供的信息。然而，通过使用来自其他生物体的序列的保守区域，围绕感兴趣的外显子，可以获得对可能发生功能重要的SNP的区域的额外了解。将该信息与重复掩蔽和图形成像相结合允许快速视觉检测新的SNP搜索空间，具有成功PCR扩增的良好前景。我们现在正在通过自动检索和处理350个感兴趣的膜转运蛋白的信息来扩展先前的试点研究，以探索这一想法。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Previously, the NIH/GM-funded Pharmacogenetics of Membrane Transporters (PMT) research project restricted areas of single nucleotide polymorphism (SNP) discovery in membrane transporters to exons and short flanking sequences surrounding these exons, resulting in little leveraging of information available from other genome projects. However, by using conserved regions of sequence from other organisms surrounding the exon of interest additional insight can be gained into areas where functionally important SNPs may occur. Combining this information with repeat masking and graphical imaging allows for rapid visual detection of new SNP search spaces with a good prospect of successful PCR amplification. We are now extending a previous pilot study exploring this idea by automating the retrieval and processing of this information for 350 membrane transporters of interest.