CRYSTAL STRUCTURE OF THE RIBOSOME

这个子项目是利用由NIH/NCRR资助的中心拨款提供的资源的许多研究子项目之一。子项目和调查员(PI)可能从另一个NIH来源获得了主要资金，因此可能会出现在其他CRISE条目中。列出的机构是针对中心的，而不一定是针对调查员的机构。我们的目标是：(A)提高70年代核糖体晶体的分辨率，目前限制在5.0°；(B)获得更多核糖体功能复合体的晶体结构，包含不同功能状态下的mRNA、tRNA和翻译因子。我们首先计划筛选我们最近获得的数十个70年代核糖体和核糖体功能复合体的晶体，包括至少两种不同晶型的晶体。如果我们在筛选过程中观察到有希望的衍射结果，我们将根据可用的时间收集数据。我们有必要在同步加速器上进行筛选，因为我们的单胞尺寸很大，因此衍射很弱。出于同样的原因，我们更喜欢使用高通量的光束线，并使用大型探测器，以获得足够的强度和光斑分离。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Our goals are to (a) obtain improved resolution of crystals of 70S ribosomes, currently limited to 5.0 ¿¿ and (b) to obtain crystal structures of additional ribosome functional complexes, containing mRNA, tRNAs and translational factors in various functional states. We first plan to screen several dozen crystals of 70S ribosomes and ribosome functional complexes that we have recently obtained, including ones from at least two distinct crystal forms. If we observe promising diffraction results during screening, we will collect data, according to the time available. It is necessary for us to carry out screening at the synchrotron because of the very large size of our unit cells, and consequent weak diffraction. For the same reason, we prefer to use beamlines with high flux, and using large detectors, to obtain adequate intensities and spot separation.