CRYSTAL STRUCTURE OF THE RIBOSOME

这个项目的长期目标是了解分子 从翻译的三维结构看翻译机制 核糖体及其与其功能配体的相互作用。这个 目的是确定嗜热嗜热菌70的结构 核糖体，在与mRNA，tRNA和翻译因子的复合体中 平移伸长循环的不同状态。最直接的 目标是研究几种功能性的共晶配合物在介质(约. 9埃单位)分辨率。更长期的计划是将重点放在 最有希望在更高的位置上解决问题(约3 Angstrom 单位)分辨率。预计这些结果不仅将 为理解这一机制提供了良好的结构基础 在分子水平上的翻译，也将揭示丰富的 关于RNA结构和蛋白质-RNA相互作用的一般信息。

The long-term goal of this project is to understand the molecular mechanism of translation in terms of the three-dimensional structure of the ribosome and its interactions with its functional ligands. The purpose is to determine the structure of Thermus thermophilus 70S ribosomes, in complex with mRNA, tRNAs and translational factors in different states of the translational elongation cycle. The immediate goal is to study several functional co-crystal complexes at medium (ca. 9Angstrom units) resolution. The longer-term plan is to then focus on the most promising candidate for solution at much higher (ca. 3Angstrom units) resolution. It is expected that these results will not only provide a sound structural basis for understanding the mechanism of translation at the molecular level, but will also reveal a wealth of information about RNA structure and protein-RNA interactions in general.