FACTORS IMPORTANT FOR PATHOGENICITY IN CAMPYLOBACTER JEJUNI: BIOTERRORISM

本子项目是利用由NIH/NCRR资助的中心赠款提供的资源的众多研究子项目之一。子项目和研究者（PI）可能已经从另一个NIH来源获得了主要资金，因此可以在其他CRISP条目中表示。列出的机构是中心的，不一定是研究者的机构。空肠弯曲杆菌是一种重要的新出现的细菌性食源性病原体，可引起一系列疾病，包括胃肠炎、直肠炎、败血症、流产、脑膜炎和上升性麻痹。家禽、猪肉、牛肉或其他食物和水中的800种微生物就能致病。每1000名接触者中就有1人患上衰弱的神经系统疾病。关于空肠梭菌感染形成的分子机制知之甚少。家畜是人类细菌的重要来源，但从动物到人类的转移机制尚不完全清楚。鸡、牛和猪作为胃肠道共生体携带空肠梭菌，很少因该细菌而发生临床疾病和病理。我们研究的长期目标是从食物链中消除弯曲杆菌。该项目的长期目标是开发蛋白质组学方法，为密歇根州立大学研究界、兽医界、食用动物生产者、美国农业部、医学界和美国生物恐怖主义研究提供资源。我们将与我们在中心的合作者合作，建立使用自动化高通量二维电泳方法分型弯曲杆菌分离物的方法。我们收集了2500多株从动物和人类身上分离的弯曲杆菌，以及从正常动物和环境中分离的弯曲杆菌。将建立其中一些菌株的蛋白表达谱。一旦生成，将对模式进行比较，并探索独特的蛋白质的身份和功能，并生成一个数据库。产生的信息将产生许多应用，但我们将侧重于确定引起疾病的分离株共有的毒力属性，而非致病性共生体中不存在毒力属性。从长远来看，我们将开发致病性空肠梭菌分离株的概况，这可以作为在疫情暴发和其他应用中表征分离株的快速筛选。这将作为利用蛋白质组学技术开发病原微生物自动快速筛选的示范项目。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Campylobacter jejuni is a significant emerging bacterial food borne pathogen of humans that causes a spectrum of diseases including gastroenteritis, proctitis, septicemia, abortion, meningitis, and ascending paralysis. As few as 800 organisms in poultry, pork, beef or other foods and water can cause disease. 1 out of 1000 exposed people develop debilitating neurological disease. Little is known about the molecular mechanisms involved in establishment of a C. jejuni infection. Domestic animals are a significant source of the bacterium for humans, but the mechanisms of transfer from animals to humans are not fully understood. Chickens, cattle and pigs carry C. jejuni as a GI commensal and only rarely develop clinical disease and pathology due to the bacterium. The long-term goal of our research is to eliminate Campylobacter from the food chain. The long-term goal of this project is to develop proteomic methods that could serve as a resource for the MSU research community, the veterinary community, food animal producers, USDA, the medical community, and US bioterrorism research. We will work with our collaborators in the Center to establish methods for typing Campylobacter isolates using automated high throughput 2 dimensional electrophoresis methods. We have assembled over 2500 isolates from animals and humans with disease due to Campylobacter, as well as isolates from normal animals and the environment. Protein expression profiles will be established for some of these strains. Once generated, patterns will be compared and unique proteins explored for identity and function, and a database will be generated. Numerous applications will arise from the information generated, but we will focus on identifying virulence attributes shared by isolates causing disease and not present in nonpathogenic commensals. In the long term we will develop a profile for pathogenic C. jejuni isolates that could serve as a rapid screen for characterizing isolates in outbreaks and other applications. This will serve as a demonstration project for developing automated rapid screens for pathogenic microorganisms using proteomics.