Development of new tools and resources to enhance wheat functional genomics

我们建议改进和利用最近开发的技术，这些技术利用有缺陷的错配修复基因来创建包含高频随机突变的小麦植株集合，这些突变可以通过直接序列分析或基于耕作的程序进行筛选。这一收集将是英国小麦社区的宝贵资源，因为它将代表农学上重要基因的新的和新的等位基因的来源。我们建议向整个小麦社区开放资源，作为一种不受多边贸易协定制约的资源，并遵循知识产权协议。该程序利用转基因技术创造新的等位基因，然而，任何新的或有用的等位基因都可以通过传统的杂交和分离从转基因中很容易地分离出来。

We propose to improve and exploit recently developed technologies, which utilise defective mismatch repair genes to create a collection of wheat plants containing a high frequency of random mutations that can be screened either by direct sequence analysis or TILLING-based procedures. This collection will be a valuable resource for the UK wheat community, as it would represent a source of new and novel alleles for agronomically important genes. We propose to open up the resource to the entire wheat community as an unencumbered resource free of MTAs and follow on IP agreements. The procedure utilises transgenic technology to create new alleles, however, any novel or useful alleles identified can easily be separated from the transgene by conventional crossing and segregation.