DIET, EXERCISE, AND METABOLISM IN OLDER WOMEN (DEMO)

本子项目是利用由NIH/NCRR资助的中心赠款提供的资源的众多研究子项目之一。子项目和研究者（PI）可能已经从另一个NIH来源获得了主要资金，因此可以在其他CRISP条目中表示。列出的机构是中心的，不一定是研究者的机构。当有氧运动（AEX）强度与低热量饮食（diet）相结合时，可能是影响选择性腹部脂肪减少的重要因素。本研究的目的是确定AEX强度影响绝经后腹部肥胖妇女在同等能量不足条件下腹部脂肪组织损失的细胞机制。假设是，与单独的DIET或低强度AEX的DIET相比，高强度AEX的DIET将增加腹部脂肪的损失和代谢心血管疾病（CVD）危险因素的改善，因为脂蛋白脂肪酶（LPL）活性的更大降低，以及腹部脂肪分解（相对于臀脂肪组织）的更大增加。我们还将测量可能参与区域脂肪储存调节的基因的信使核糖核酸（mRNA）水平，以开始确定有和没有不同强度AEX的饮食如何影响基因表达，从而改变身体脂肪分布。确定有或没有不同强度AEX的饮食影响甘油三酯的区域摄取和动员以改变体脂肪分布的机制，对于开发最有效的治疗方法来促进腹部脂肪的优先减少和改善腹部肥胖女性心血管疾病代谢危险因素具有重要的临床意义。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Aerobic exercise (AEX) intensity may be an important factor affecting the selective loss of abdominal fat when combined with a hypocaloric diet (DIET). The goal of this study is to determine the cellular mechanisms by which AEX intensity affects the loss of abdominal adipose tissue under conditions of equal energy deficit in postmenopausal women with abdominal obesity. The hypothesis is that, compared to DIET alone or DIET with low-intensity AEX, DIET with high-intensity AEX will augment the loss of abdominal fat and improvements in metabolic cardiovascular disease (CVD) risk factors due to greater reductions in lipoprotein lipase (LPL) activity, and to greater increases in lipolysis, in abdominal, relative to gluteal, adipose tissue. We also will measure messenger ribonucleic acid (mRNA) levels of genes that may be involved in the regulation of regional fat storage to begin to determine how DIET with and without AEX of different intensities affects gene expression to alter body fat distribution. Identification of the mechanisms by which DIET with and without AEX of different intensities affects regional uptake and mobilization of triglyceride to alter body fat distribution will have important clinical implications for the development of the most effective treatment to promote the preferential loss of abdominal fat and improve CVD metabolic risk factors in women with abdominal obesity.