INTERACTIONS IN PCOS

这个子项目是利用由NIH/NCRR资助的中心拨款提供的资源的许多研究子项目之一。子项目和调查员(PI)可能从另一个NIH来源获得了主要资金，因此可能会出现在其他CRISE条目中。列出的机构是针对中心的，而不一定是针对调查员的机构。其具体目的是验证17b-HSD5基因激活变异体的表型取决于与黄体生成素、胰岛素抵抗高胰岛素血症和卵泡刺激素依赖颗粒细胞因子的相互作用的假设。我们将首先确定PCOS患者和对照组已知的17b-HSD5基因女性对促黄体生成素类似物人慢性促性腺激素(HCG)的睾酮反应。然后，我们将确定用促性腺激素释放激素激动剂抑制促性腺激素，用噻唑烷二酮(TZD)抑制胰岛素，以及用FSH刺激颗粒细胞功能的效果。此外，还将评估TZD抑制胰岛素对黄体生成素脉搏特征和胰岛素分泌/敏感性指数的影响。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The specific aim is to test the hypothesis that the phenotype of an activating variant of the 17b-HSD5 gene depends on interactions with LH, insulin resistant hyperinsulinemia, and FSH dependent granulosa cell factors. We will begin by determining the testosterone response to the LH analog human chronic gonadotropin (hCG) in PCOS and control women of known 17b-HSD5 genotype. Then we will determine the effects of suppressing gonadotropins with a GnRH agonist, suppressing insulin with a thiazolidinedione (TZD), and stimulating granulosa cell function with FSH. The effects of suppressing insulin with a TZD on LH pulse characteristics and indexes of insulin secretion/sensitivity will also be assessed.