CORE B PROTEIN, NUCLEIC ACID ANALYSIS AND CELL IMAGING

该子项目是利用NIH/NCRR资助的中心赠款提供的资源的许多研究子项目之一。子项目和研究者（PI）可能从另一个NIH来源获得主要资金，因此可以在其他CRISP条目中表示。所列机构为中心，不一定是研究者所在机构。在上一个资助期间，该核心为与该COBRE相关的所有研究人员以及来自MMCRI和外部机构的许多其他研究人员提供了DNA测序，蛋白质测序，用于分析和鉴定蛋白质的质谱分析，以及共聚焦显微镜资源。对核心服务的需求随着机构的发展而增长。 雇用更多在使用高端仪器（质谱仪、共聚焦显微镜）和解释这些仪器产生的数据方面有经验的人员，已纳入未来五年COBRE支助的预算和研究计划。这将包括雇用2名全职人员和增加D。斯派塞担任核心的联席董事。D.斯派塞已被添加到核心，以协调内部和外部的微阵列研究。核心委员会为所提供的服务制定了收费服务表。这些费用将补充COBRE和机构资金，以帮助满足对核心服务日益增长的需求，并在COBRE资源不再可用时使核心实现自给自足。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. During the previous funding period this Core provided DNA sequencing, protein sequencing, mass spectrometry for analysis and identification of proteins, and confocal microscopy resources to all investigators associated with this COBRE and many other investigators from both MMCRI and from outside institutions. The demand for Core services has grown with the growth of the Institution. The hiring of additional personnel experienced in the use of high end instrumentation (mass spectrometry, confocal microscopy) and interpretation of the data generated by these instruments has been incorporated into the budget and research plan for the next five years of COBRE support. This will include hiring 2 FTEs and the addition of D. Spicer as a Co-Director of the Core. D. Spicer has been added to the Core to coordinate both internal and external microarray studies. The Core has established a fee-for-service schedule for services offered. These fees will supplement COBRE and Institutional funds to help meet the increasing demand for Core services as well as to move the Cores towards self-sufficiency when COBRE resources are no longer available.