DETERMINING AQUATIC BIOINDICATORS FOR ATRAZINE

本子项目是利用由NIH/NCRR资助的中心赠款提供的资源的众多研究子项目之一。子项目和研究者（PI）可能已经从另一个NIH来源获得了主要资金，因此可以在其他CRISP条目中表示。列出的机构是中心的，不一定是研究者的机构。谢恩河和野生稻河中特定大型无脊椎动物的发生与阿特拉津水平相关。目前正在开发一种高效液相色谱法，用于将阿特拉津及其代谢物定量到十亿分之一（ppb）。酶联免疫吸附试验（ELISA）试剂盒最近获得了EPA的批准。环境技术验证（ETV）程序用于检测ppb水平的阿特拉津。我们打算使用试剂盒作为筛选，然后通过高效液相色谱进行精确测定。我们的第二个具体目标是确定两个河流系统中的大型无脊椎动物，并将它们的出现与阿特拉津水平联系起来。8月下旬，我们在野生水稻上的6个地点进行了大型无脊椎动物取样，收集了常见的摇蚊科（蠓）近300只。所有个体都被鉴定为至少属水平的光学显微镜。基于前人对谢恩河的研究，我们初步确定了几个类群，供将来的中观实验使用。这些包括Chironomids Polypedilum， Cryptochironomus， Chironomus和Procladius；五月蝇（hexenia limbata和Caenis）；球童的水心理；甲虫Dubiraphia；以及片足类动物阿兹特克海莱拉。我们已经获得了过去几年在谢延河流域使用阿特拉津的GIS地图，并正在等待其在野生稻流域的使用地图。今年，我们将在我们的两个河流流域进行严格的阿特拉津抽样制度。此外，我们将继续对这些河流中的大型无脊椎动物进行取样和监测，并试图确定与阿特拉津水平的相关性。第二年的一项主要任务将是购买和建立第一批mescomms，或人工溪流。我们希望开始我们的第三个具体目标的初步研究，以检查任何可能的内分泌影响的大型无脊椎动物长大的中胚层。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The occurrence specific macroinvertebrates in the Sheyenne and Wild Rice rivers will be correlated with Atrazine levels. An HPLC protocol is being developed for the quantitation of Atrazine and its metabolites to parts per billion (ppb). Enzyme-Linked Immunosorbent Assay (ELISA) kits have recently been approved by the EPA?s Environmental Technology Verification (ETV) program for use in detecting Atrazine in the ppb level. We intend to use the kits as a screen, follwed by precise determination via HPLC. Our second specific aim is to identify the macroinvertebrates in the two river systems and correlate their occurrence with Atrazine levels. We carried out macroinvertebrate sampling on six sites on the Wild Rice in late August, collecting approximately 300 individuals from the commonly occuring family Chironomidae, (midges). All individuals were identified to at least the genus level using light microscopy. Based on previous studies on the Sheyenne River we have tentatively identified several taxa for future mesocosm experiments. These include the Chironomids Polypedilum, Cryptochironomus, Chironomus, and Procladius; the mayflies Hexagenia limbata and Caenis; the caddisfly Hydropsyche; the beetle Dubiraphia; and the amphipod Hyallela azteca. We have obtained GIS mapping of Atrazine usage in the Sheyenne River basin over the last several years and are waiting for maps of its usage in the Wild Rice basin. This year we will carry out a rigorous sampling regime for Atrazine in our two river basins. In addition we will continue to sample and monitor the macroinvertbrate fauna in those rivers and try and identify correlations with Atrazine levels. A major undertaking in the second year will be the purchase and set up of the first set of mescosms, or artificial streams. We hope to begin preliminary studies for our third specific aim, to examine any possible endocrine effects on macroinvertebrates raised in the mesocosms.