LSU DENTAL COBRE: ORAL HUMAN PAPILLOMAVIRUS INFECTION IN HIV-POSITIVE PATIENTS

这个子项目是利用由NIH/NCRR资助的中心拨款提供的资源的许多研究子项目之一。子项目和调查员(PI)可能从另一个NIH来源获得了主要资金，因此可能会出现在其他CRISE条目中。列出的机构是针对中心的，而不一定是针对调查员的机构。前言：通过高效抗逆转录病毒治疗(HAART)，HIV阳性患者的全身免疫重建得以实现，许多口腔感染也随之减少。然而，最近的报告指出，与人类乳头瘤病毒(HPV)感染相一致的口腔病理增加，HPV是一种在艾滋病毒阳性患者的口腔和生殖道中发现的病毒。该项目的目标是评估HAART对HIV阳性个体口腔中HPV感染的影响。为了实现这一目标，将使用纵向收集的口腔拭子、唾液和血液以及病变阳性组织来检查艾滋病毒阳性个人对HPV的局部和系统免疫反应。目的：招募受试者参加这项临床研究；建立实验室，配备必要的样本分析设备；接受科学研究人员的教育。方法：与指导团队合作，明确患者入选和分层，建立实验室；参加科布雷教育活动。结果：从第一年开始：受试者已经入选，采集口腔拭子，采集唾液和血液。已从拭子和唾液细胞小球中提取DNA，以检测是否存在HPV，并将唾液上清液冷冻，以供将来检测细胞因子和HPV抗体。已经聘请了一名临床协调员0.5FTE，一名研究助理(0.5FTE)和一名学生工作者。PI正在与研究助理合作，以优化该项目所需的实验室技术。PPI参加了Cobre主办的月刊俱乐部、寻求赠款的会议、全面的临床培训课程、进展报告和陈述，以及参加NIH格兰特作家研讨会、在巴尔的摩举行的2005年IADR/AADR年会和在温哥华举行的第22届国际乳头状瘤病毒会议。结论：在指导团队的支持下，PI有望成为一名有能力获得独立资助的临床研究人员。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Introduction: Systemic immune reconstitution for the HIV-positive patient has been accomplished with highly active anti-retroviral therapy (HAART) with an associated decrease in many oral infections. However recent reports have noted increases in oral pathology consistent with human papillomavirus (HPV) infection, a virus found in both oral and genital tracts of HIV-positive patients. The goal of this project is to evaluate the impact of HAART on HPV infection in the oral cavity of HIV-positive individuals. To achieve this, longitudinally collected oral swabs, saliva and blood as well as lesion-positive tissue, will be used to examine local and systemic immune responses to HPV in HIV-positive individuals. Objective: To enroll subjects into this clinical study; to set-up a lab with the necessary equipment for sample analysis; to become educated as a scientific investigator. Methods: Working with the mentoring team to clarify patient enrollment and stratification and set up the lab; attend COBRE educational activities. Results: The following is progress from year 1: Subjects have been enrolled, oral swabs obtained, saliva and blood collected. DNA has been extracted from the swabs and salivary cell pellet to test for the presence of HPV and the saliva supernatant frozen for future cytokine and HPV antibody detection. A clinical coordinator 0.5 FTE, a research associate (0.5 FTE) and a student worker have been hired. The PI is working with the research associate to optimize the laboratory techniques necessary for this project. The PI participated in COBRE sponsored monthly journal club, grant seeker sessions, comprehensive clinical training course, progress reports and presentations as well as attending an NIH grantwriters workshop, the 2005 IADR/AADR annual meeting in Baltimore and the 22nd International Papillomavirus Conference in Vancouver. Conclusion: The PI with support from the mentoring team is poised to become a clinical researcher capable to obtaining independent funding.