COBRE:RW HOSP: P1: POTENTIAL OF SKIN STEM CELLS AND WOUND HEALING

本子项目是利用由NIH/NCRR资助的中心赠款提供的资源的众多研究子项目之一。子项目和研究者（PI）可能已经从另一个NIH来源获得了主要资金，因此可以在其他CRISP条目中表示。列出的机构是中心的，不一定是研究者的机构。最近的研究发现，来自特定器官的干细胞能够在其他器官中产生成熟组织，这改变了我们对干细胞在人类疾病和发育中的作用的看法。骨髓干细胞是最具特征的干细胞之一，它们已被证明能够熟练地形成非血液组织。骨髓很可能为许多器官提供细胞基质，并且骨髓衍生祖细胞被招募到损伤或伤口区域。有证据表明肝脏和心脏也是如此。了解这些骨髓细胞归巢和募集到皮肤的动力学将对开发各种疾病的治疗方法有价值。我们已经在初步研究中表明，骨髓细胞可以移植到伤口中并形成皮肤结构。在一项对人类患者进行的临床研究中，我们有证据表明，骨髓可以加速愈合和关闭那些在专业中心进行的其他传统和先进治疗无法愈合的慢性伤口。所提出的研究将确定那些进入皮肤并形成皮肤结构的骨髓干细胞。纯化的骨髓干细胞和整个骨髓将被检查其移植和重建受伤皮肤结构的潜力。利用这些成体干细胞的能力也可能使我们避免使用不可预测的胚胎干细胞，并可能形成未来所有干细胞治疗的基础。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Recent findings that stem cells from a particular organ are capable of giving rise to mature tissues in other organs have changed our thinking about the role of stem cells in human disease and development. Bone marrow stem cells are among the most well characterized stem cells and they have been shown to be proficient in forming non-blood tissues. It is likely that bone marrow provides the cellular substrates for many organs and that bone marrow derived progenitors are recruited to areas of injury or wounds. There is evidence of this for the liver and heart. Understanding the dynamics of homing and recruitment of these bone marrow cells to the skin will be valuable in developing treatments for a variety of disorders. We have shown in preliminary studies that bone marrow cells can engraft into wounds and form skin structures. In a clinical study performed on human patients, we have evidence that bone marrow can accelerate healing and close chronic wounds that have failed to heal by other traditional and advanced treatments administered in specialized centers. The studies proposed will identify those bone marrow stem cells that home to skin and form skin structures. Purified bone marrow stem cells and whole bone marrow will be examined for their potential to engraft and reconstitute skin structures in wounded skin. The ability to utilize these adult stem cells may also allow us to avoid the use of unpredictable embryonic derived stem cells and may form the basis for all stem cell based treatments in the future.