Center for Dynamic Imaging of Nervous System Function

在生命科学领域，人们经常断言，我们并不缺乏问题;相反，我们受到解决这些问题的技术可用性的限制。在过去十年中，开发了一些新技术来解决生物问题。然而，使用这些新开发的技术的障碍往往是相当大的个人调查。该中心的目标是提供一个集中的和配备人员的设施，让专家和新手在他们的研究中使用最现代的研究工具，并配备这些工具。 用于生理学研究的动态成像仪器。我们将围绕三个核心开发一个中心：动态成像核心，包含两个双光子显微镜，光谱检测共聚焦显微镜，全内反射荧光显微镜以及光解附件;数据分析核心，将为中心显微镜收集的所有数据提供离线分析的机会，以及为中心动态成像核心的新老用户提供咨询核心。 该中心的目标分为三个科学集群：I）神经元发育，其中OT 项目将研究从干细胞生物学到神经元迁移和突触形成的问题， II）离子通道和突触传递的调节，其中我们的研究包括控制钙通道活性的蛋白质-蛋白质相互作用、受体的空间分布和控制神经元网络的突触的定位，以及III）神经胶质-神经元相互作用，其中我们将研究星形胶质细胞中的钙信号传导、离子通道在髓鞘形成中的作用以及神经胶质释放的信使在突触形成中的作用。 建立这个动态成像中心将促进新的研究，并增加现有的研究在21个合格的N1 NDS资助的实验室（包括34个N1 NDS赠款），包括这个中心。由此产生的教师之间的协同作用将彻底改变我们的能力，研究神经系统功能的基本功能，并将最终提高我们对这些功能在人类健康和疾病的理解。

It is often averred in the Life Sciences that we are not short of questions; rather, we are limited by the availability of technologies to address these questions. During the past decade several new technologies have been developed to address biological problems. However, barriers to using these newly-developed technologies are often considerable for the individual investigator. The goal of this Center is to provide a centralized and staffed facility to allow both the expert and the novice to use the most modern research tools in their studies, and to equip these instruments for dynamic imaging in physiological studies. We will develop a Center around three cores Dynamic Imaging Core, containing, two two-photon microscopes, spectral detection confocal microscopes, total internal reflectance fluorescence microscope together with photolysis attachments; Data Analysis Core that will provide opportunities for off-line analysis of all data collected with the Center's microscopes, and Consultation Core for new and established users of the Center's Dynamic Imaging Core. The goals of this Center fall into three scientific clusters: I) Neuronal Development, in which ot projects will investigate problems ranging from stem cell biology to neuronal migration and synapse formation, lI) Regulation of Ion Channels and Synaptie Transmission, in which our studies include protein-protein interactions controlling calcium channel activity, the spatial distribution of receptors, and the localization of synapses controlling neuronal networks, and Ill) Gila-Neuron Interactions, in which we will investigate calcium signaling in astrocytes, the roles of ion channels in myelination, and the role of glial-released messengers in synapse formation. Establishing this Dynamic Imaging Center will catalyze new studies and augment existing studies in the 21 qualifying N1NDS-funded laboratories (including 34 N1NDS grants) that comprise this Center. The resultant synergistic interactions between faculty members will revolutionize our ability to study basic functions of the nervous system function and will ultimately enhance our understanding of these functions in human health and disease.