Nanoparticle Based Magnetic Microfluidic concentrator (MMC)

描述（由申请人提供）：富集的异常脱落细胞和生物分子将使基因组学、蛋白质组学和表观基因组学分析能够检测表达基因、蛋白质/肽谱和表观遗传标记物中的癌症特异性改变。使用体液以常规方式进行这种富集和分析的能力可能导致癌症检测的改进。 NIH SBIR的第一阶段提案是开发一种高通量和高富集因子系统，用于富集稀有癌细胞和细胞碎片。在第一阶段，将通过我们的模型系统研究癌细胞、癌蛋白和所有DNA的富集。在第二阶段，将使用体液中的癌细胞和细胞碎片来优化该系统。最终交付物将包括MMC、抗体固定化MNP和所有必要的方案。

DESCRIPTION (provided by applicant): Enriched abnormal exfoliated cells and biomolecules will enable genomic, proteomic, and epigenomic analyses to detect cancer-specific alterations in expressed genes, protein/peptide profiles, and epigenetic markers. The ability to carry out such enrichment and analyses in a routine way using body fluids may lead to improvements in cancer detection. This NIH SBIR Phase I proposal is to develop a high throughput and high enrichment factor system for the enrichment of rare cancer cells and cell fragments. In phase I, the enrichment of cancer cell, cancer protein and all DNAs will be studied through our model system. In phase II, the cancer cells and cell fragment in body fluids will be used to optimize this system. The final deliverables will include a MMC, antibody immobilized MNPs and all necessary protocols.