Cell Imaging, Analysis and Microinjection

细胞成像、分析和微注射核心(CIAMC)包含有价值的现代工具， 作为常规服务提供给计划项目成员。所提供的服务对 个别实验室项目和方案内研究。中国汽车工业协会是在原 应用程序，以满足这一领域调查人员日益增长的需求。CIAMC已经演变到现在 摩尔UCSD数字成像共享资源(DISR)中显微镜资源的优势。我们 在此申请中建议资助一名兼职研究助理，为PPG提供专门的支持 成员通过1)提供使用这些三维荧光的标准化培训。显微镜和 体细胞显微注射系统，2)在该PPG内训练个体以充分利用 我们组中提供了增强的科学可视化软件，以及3)提供有关使用的实际帮助 这些显微镜。我们设施的一个独特之处是它与VisLab的计算机科学家建立了合作关系 在圣地亚哥超级计算机中心(SDSC)。他们创造了用于增强3-D的新工具 细胞的可视化，基于Web的数据处理，并利用了几乎无限的 SDSC的计算能力，有助于存储和分析超大型数据集 (http://vis.sdsc.edu/research/cancer.html).)随着频率的增加，目前的PPG调查人员正在 使用这些增强的功能，拟议的CIAMC将促进PPG成员的能力 及时访问这些服务。

The Cell Imaging, Analysis, & Microinjection Core (CIAMC) comprises valuable, modern tools that are made available as routine services to Program Project members. The services provided have been crucial for individual laboratory projects, and intra-programmatic research. The CIAMC was established in the original application to meet the growing needs of the investigators in this area. The CIAMC has evolved to now take advantage of microscopy resources in the Moores UCSD Digital Imaging Shared Resource (DISR). We propose in this application to fund a part-time research assistant to provide dedicated support to PPG members by 1) providing standardized training in the use of these 3-D fluorescence.microscopes and the somatic cell microinjection system, 2) training individuals within this PPG to take full advantage of the enhanced scientific visualization software available in our group, and 3) providing hands-on help with the use of these microscopes. A unique feature of our facility is its partnership with computer scientists at the VisLab in the San Diego Supercomputer Center (SDSC). They have created new tools for enhanced 3-D visualization of cells, web-based data processing, and have made use of the virtually unlimited computational power of the SDSC to facilitate storage and analysis of very large data sets (http://vis.sdsc.edu/research/cancer.html). With increasing frequency, current PPG investigators are making use of these enhanced features, and the proposed CIAMC will facilitate the ability of PPG members to access these services in a timely manner.