Core-Electron Spin Resonance

核心A的目的是为PPG参与者提供最先进的血管细胞和组织中的活性氧（ROS）和一氧化氮（NO）测量协助。CORE A将协助制备和处理用于电子自旋共振（ESR）光谱分析的各种生物样品。具有自旋捕获技术的ESR光谱是一种最直接和明确的方法，即使在使用少量生物材料的强散射介质中也能进行高灵敏度的活性氧定量检测。该核心设施自1999年以来一直支持超氧自由基（02.-）和NO的研究。在血管细胞的一些出版物中。

The purpose of Core A is to provide assistance with state-of-the-art measurements of reactive oxygen species (ROS) and nitric oxide (NO.) in vascular cells and tissues for the participants of the PPG. CORE A will assist with preparation and processing of various biological samples for analysis using Electron Spin Resonance (ESR) spectroscopy. ESR spectroscopy with a spin trapping technique is one of the most direct and unequivocal methods for high sensitivity quantitative detection of reactive oxygen species even in strongly scattering media using minimal amounts of biological material. This Core facility has existed since 1999 and has supported studies of superoxide radical (02.-) and NO. in vascular cells in several publications from Drs. Griendling and Harrison (1- 10). This facility will significantly enhance the work of the individual labs and will clearly improve collaborations between participants of the PPG. Specifically, the Electron Spin Resonance Core will: 1. Offer expertise, facilities, and training for individuals in performing measurements of ROS and NO. using ESR spectroscopy. 2. Provide assistance with state-of-the-art in vitro and ex vivo measurements of ROS and NO. in vascular cells and tissues for participants of the PPG. 3. Develop techniques and refine existing methods for measurement of ROS and NO. in vascular tissues.