Core--Histology

罗切斯特大学心血管研究中心（CCVR）已经认识到 需要多用户设施核心，旨在为CCVR调查人员提供专业知识、服务和 仪器，以加强正在进行的研究计划，并促进新的研究工作。通过满足 以统一的方式满足CCVR调查人员的需求，我们可以在人员工作和 设备利用率，并在许多情况下，提供服务和研究投入无法获得的个人 调查员因此，组织学核心将提供所有基础设施和技术支持，以修复，嵌入，切片， 并对动物组织进行常规染色和组织形态计量学处理， 免疫组织化学和原位杂交（整体封片和载玻片）。核心将建立一个 数据库进行幻灯片整理，必要时进行数据解释。在这种情况下，所有项目负责人将 可以立即访问每个项目内的数据，这应有助于规划和/或修改 随后的研究。核心还将作为向每个项目的关键技术转让的培训基地。 工作人员例如，所有的基础科学项目都建议在LDLR-/-小鼠中进行研究，其中 将进行组织形态测定以评估各种转基因对斑块面积的影响。组织形态测定的 分析需要大量的技术时间，这将侵犯组织处理和冲压所需的时间， 公里.我们预计，通过将这项技术转让给每个项目下的工作人员，组织学 核心设施将大大减轻。这样做的好处是最大限度地减少雇用额外的 组织学核心内的人员，同时在每个项目负责人的指导下最大限度地利用技术人员。

The University of Rochester Center for Cardiovascular Research (CCVR) has recognized the need for multi-user Facility Cores designed to furnish CCVR investigators with expertise, service and instrumentation to enhance ongoing research programs and to facilitate new research endeavors. By meeting the needs of the CCVR investigators in a consolidated fashion, we can be more efficient in personnel effort and in equipment utilization and, in many cases, provide both service and research input unavailable to the individual investigator. Accordingly, the Histology Core will provide all infrastructure and technical support to fix, embed, section, and process animal tissues for routine staining and histomorphometry as well as such specialized methods as immunohistochemistry and in situ hybridization (both whole mount and slide). The Core will institute a database for slide collation and, when necessary, data interpretation. In this manner, all Project leaders will have immediate access to data within each project, which should facilitate the planning and/or modification of subsequent studies. The Core will also serve as a training ground for key technology transfer to each Project's staff. For example, all of the basic science projects propose studies in the LDLR-/- mouse wherein histomorphometry will be done to assess the effects of various transgenes on plaque area. Histomophometric analyses require a lot of tech time, which would infringe on time needed for tissue processing and stamping to slides. We anticipate that by transferring this technology to staff under each project, the workload within the Histology Core facility will be lightened significantly. This has the advantage of minimizing the hiring of additional personnel within the Histology Core, while maximizing the use of technical staff under each Project leader's direction.