Mitochondrial Dysfunction in Aging and Diseses

描述(申请人提供)：与年龄相关的人类疾病，包括癌症、阿尔茨海默氏症和帕金森氏病，显示出与线粒体功能障碍和活性氧物种(ROS)明显相关。我们建议采用遗传、生化和生物能量学相结合的方法来检验线粒体功能障碍，特别是线粒体活性氧物种(ROS)的产生与年龄相关疾病的病因性参与的假设。我们建议使用线粒体功能的三个主要操作-电子传输链复合体、谷胱甘肽池和超氧化物水平-并测量这三个与年龄相关的疾病的结果模型。 我们相信，拟议的计划项目将对我们理解线粒体功能如何导致年龄相关疾病做出重大贡献，以期设计成功的干预措施。

DESCRIPTION (provided by applicant): Age-related human diseases including cancer, Alzheimer's and Parkinson's disease show a clear correlation with mitochondrial dysfunction and reactive oxygen species (ROS). We propose to undertake a combined genetic, biochemical and bioenergetics approach to test the hypothesis that mitochondrial dysfunction and particularly mitochondrial reactive oxygen species (ROS) generation are causatively involved in age related disease. We propose to use three main manipulations of mitochondrial features-electron transport chain complexes, the glutathione pool and superoxide levels-and to measure outcomes models of these three age-related conditions. We believe that the proposed Program Project will make a significant contribution to our understanding of how mitochondrial function contributes to age-related disease with a view towards designing successful interventions.