Mitochondrial Dysfunction in Aging and Disease

描述（由申请人提供）：与线粒体功能障碍和活性氧（ROS）明显相关的人类疾病，包括癌症、阿尔茨海默病和帕金森病。我们建议采取一个综合的遗传，生物化学和生物能量学的方法来测试的假设，线粒体功能障碍，特别是线粒体活性氧（ROS）的产生是因果关系参与年龄相关疾病。我们建议使用三个主要的操纵线粒体功能电子传递链复合物，谷胱甘肽池和超氧化物水平，并测量这三个年龄相关的条件的结果模型。 我们相信，拟议的计划项目将为我们理解线粒体功能如何有助于年龄相关疾病做出重大贡献，以期设计成功的干预措施。

DESCRIPTION (provided by applicant): Age-related human diseases including cancer, Alzheimer's and Parkinson's disease show a clear correlation with mitochondrial dysfunction and reactive oxygen species (ROS). We propose to undertake a combined genetic, biochemical and bioenergetics approach to test the hypothesis that mitochondrial dysfunction and particularly mitochondrial reactive oxygen species (ROS) generation are causatively involved in age related disease. We propose to use three main manipulations of mitochondrial features-electron transport chain complexes, the glutathione pool and superoxide levels-and to measure outcomes models of these three age-related conditions. We believe that the proposed Program Project will make a significant contribution to our understanding of how mitochondrial function contributes to age-related disease with a view towards designing successful interventions.