FLOW CYTOMETRY SHARED RESOURCE

流式细胞仪共享资源为AECC调查人员提供了方便且负担得起的访问 广泛应用于流式细胞术的重要技术，并支持大量的研究 依靠高速荧光激活细胞分选(FACS)和先进的多参数 荧光分析。除了提供先进的流式细胞仪设备外，共享的资源 提供技术专业知识和培训，以指导AECC调查人员及其工作人员应用 基础和先进的流式细胞术技术。该机构还组织和支持一项 向AECC调查人员介绍FACS新应用的新技术研讨会 这可以加强他们的研究。此共享资源的主要用途是多参数分析和 基于荧光偶联抗体或其他标记的细胞群的高速分选 荧光蛋白质或化学染料试剂。对许多调查人员进行使用该设施的培训 分析仪器已经形成了一个非常庞大和训练有素的用户基础。2005-2006年间，该设施 为超过275名注册用户提供服务，其中包括教师、博士后研究员、学生和 来自78个不同实验室的技术人员。AECC成员是其中45个项目的主要调查人员 实验室，2005-2006年所有设施服务总使用时数的三分之二可归因于 AECC调查人员的实验室。这些调查人员可以全天候访问，每天七天 每周到设施的分析仪，其中包括Becton-Dickinson FACScan(一台激光，三色)，FACSCalibur (两台激光、4色)和LSR-II(4台激光、12色)仪器。标准荧光显微镜和 具有用于处理FACS数据的广泛软件库的计算机工作站也是例行公事 可用。使用该设施的3激光、8色达科细胞分选机进行高速细胞分选 仅由训练有素的设施工作人员在正常安排的工作日时间内执行。这是共享的 资源具有众多的增值功能，并得到了大量的制度支持，这些支持大大 协助收购最先进的仪器和一支优秀的流式细胞仪专家团队。

The Flow Cytometry Shared Resource provides AECC investigators with convenient and affordable access to a broad spectrum of important technologies in flow cytometry, and supports numerous research studies that rely on high speed fluorescence activated cell sorting (FACS) and advanced multiparameter fluorescence analysis. In addition to providing advanced flow cytometry equipment, the shared resource provides technical expertise and training to guide AECC investigators and their staff in the application of basic and advanced flow cytometry techniques. The facility also organizes and supports a program of seminars on new technologies that inform AECC investigators about newly emerging applications of FACS that can enhance their research. The major usage of this shared resource is for multiparameter analysis and high speed sorting of cell populations based on labeling with fluorochrome-conjugated antibodies or other fluorescent protein or chemical dye reagents. Training of many investigators in the use of the facility's analytical instruments has led to a very large and well-trained user base. During 2005-2006, the facility provided services to more than 275 registered users, consisting of faculty, postdoctoral fellows, students and technicians from 78 different laboratories. AECC members were principal investigators of 45 of these laboratories, and two thirds of total hours of usage of all facility services in 2005-2006 was attributable to the laboratories of AECC investigators. These investigators have access around the clock and seven days per week to the facility's analyzers, which include Becton-Dickinson FACScan (one laser, 3-color), FACSCalibur (two laser, 4-color), and LSR-II (4 laser, 12 color) instruments. Standard fluorescent microscopes and computer work stations with an extensive software library for processing of FACS data are also routinely available. High speed cell sorting with the facility's 3-laser, 8-color Dako-Cytomation MoFlo sorter is performed exclusively by the trained facility staff during regularly scheduled weekday hours. This shared resource has numerous value-added features, and receives substantial institutional support that has greatly assisted the acquisition of state-of-the-art instrumentation and a superb staff of flow cytometry experts.