TRANSGENIC ANIMAL

描述（由申请人提供）：转基因动物/胚胎干细胞资源共享设施是UAB综合癌症中心的重要组成部分。随着人类和小鼠基因组序列的完成，转基因小鼠模型在阐明疾病机制和开发癌症治疗方法方面比以往任何时候都更加重要。这个共享的设施为转基因小鼠模型的开发提供了独特的服务，并有长期的卓越服务记录。具体目标包括：1。提供高效，成本效益的转基因小鼠模型的生产

DESCRIPTION (provided by applicant): The Transgenic Animal/ES Cell Resource Shared Facility is an essential component of the UAB Comprehensive Cancer Center. With completion of the human and mouse genome sequences, genetically modified mouse models are more essential than ever in elucidating disease mechanisms and developing approaches to therapies for cancer. This shared facility provides unique services for the development of genetically modified mouse models and has a long track record of outstanding service. The specific aims include: 1. Provide efficient, cost effective production of transgenic mouse models for Comprehensive Cancer Center investigators by microinjection of gene constructs into fertilized mouse eggs or ES cells into blastocysts; 2. Provide additional services such as cryopreservation of embryos, embryo rederivation to produce pathogen-free mice, and assisted reproduction techniques (e.g., in vitro fertilization [IVF], superovulation, and embryo transfer); 3. Provide expert consultation services to help investigators design the most effective transgene constructs strategies for molecular diagnosis of transgenic mice, and breeding strategies required for perpetuation of transgenic mouse lines. The services provided are not available through any other mechanism except costly commercial alternatives that do not supply the complete set of services provided by this facility.