SERUM BIOMARKERS OF ALCOHOL SELF ADMINISTRATION IN NON-HUMAN PRIMATES

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 在治疗酒精滥用和酒精中毒方面，最大的障碍可能是缺乏诊断和监测过度饮酒的工具。 由于许多原因，（例如，与酗酒有关的社会耻辱，以及坦白酗酒的潜在法律的/社会后果），自我报告的使用情况虽然提供了信息，但众所周知是不一致的。 事实上，有一个动机，病人否认饮酒。 这导致了对酒精滥用的生物化学标志物的研究。 已经提出了几种酒精中毒的生化标志物，但这些检测的准确性和灵敏度都有局限性。这些生物标志物包括：平均红细胞体积、碳水化合物缺乏型转铁蛋白、γ-谷氨酰转移酶、天冬氨酸转氨酶和乙基葡糖苷酸。 本研究建议将非人灵长类动物酒精自我给药模型的能力与新的高通量蛋白质组学技术联合收割机相结合，以鉴定过量饮酒的其他诊断性血浆蛋白生物标志物特征。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Perhaps the most significant barrier in the treatment of alcohol abuse and alcoholism is the paucity of tools available for diagnosing and monitoring excessive alcohol consumption. For many reasons, (e.g., social stigma associated with alcohol abuse, and the potential legal/social ramifications of frank alcoholism), self-reported use, though informative, is known to be inconsistent. Indeed, there is a motivation for patients to deny drinking. This has led to the search for biochemical markers of alcohol abuse. Several biochemical markers of alcoholism have been proposed, but there are limitations to the accuracy and sensitivity of these assays. These biomarkers include: mean corpuscular volume, carbohydrate-deficient transferrin, gamma-glutamyl transferase, aspartate aminotransferase, and ethylglucuronide. The present study proposes to combine the power of a non-human primate model of alcohol self-administration with new, high-throughput proteomic techniques to identify additional diagnostic plasma protein biomarker signatures of excessive alcohol consumption.