DETERMINING AQUATIC BIOINDICATORS FOR ATRAZINE

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 该项目的长期目标是研究阿特拉津及其代谢物对北达科他州溪流和河流中发现的水生大型无脊椎动物的可能影响。 阿特拉津是一种常用的除草剂，在水生系统中持续存在，最近已被证明在各种生物体中起内分泌干扰物的作用。 目前在北达科他州对水生系统中的阿特拉津进行的研究很少。 此外，还需要更多关于这种除草剂对水生生物影响的数据。 流行的毒性测试方法是在受控的微观世界中进行单一物种测试，然后将结果外推到自然发生的环境中。 通常检查的物种是那些在过去的测试中很容易培养的物种，可能与当地水生生态系统中的物种没有任何关系。 这种方法因其人为性而受到批评。 我们将采取不同的方法，我们将使用现场收集的数据来确定哪些物种应考虑进行毒性试验。 在我们调查的所有阶段都将考虑当地的物种和条件。 本研究将调查水生系统中阿特拉津的几个关注领域。 它将讨论阿特拉津在水生系统中的流行情况，研究已知大型无脊椎动物的流行情况，确定可能表明阿特拉津污染的水生大型无脊椎动物，并研究阿特拉津可能对水生大型无脊椎动物产生的影响。 由于阿特拉津在哺乳动物系统中是一种内分泌干扰物，人们对接触阿特拉津可能对人类系统产生的影响表示相当关注。 阿特拉津分布广泛，是农业区地表沃茨的常见成分。 目前的水质检测程序需要进行广泛的化学分析。 发现阿特拉津污染的生物指示剂将有助于记录人类接触阿特拉津的可能途径。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The long-term goal of this project is to examine the possible effects of atrazine and its metabolites on aquatic macroinvertebrates found in streams and rivers in North Dakota. Atrazine is a commonly used herbicide that persists in aquatic systems and has recently been shown to act as an endocrine disrupter in a variety of organisms. There is currently little research being done in North Dakota on atrazine in aquatic systems. In addition, there is a need for more data on the effects of this herbicide on aquatic organisms. The popular approach to toxicity testing is to perform single species tests in a controlled microcosm and then extrapolate the results to natural occurring environments. Often the species examined are those that have been easily cultured in past testing and may not have any relationship to the species occurring in local aquatic ecosystems. This approach has been criticized because of its artificiality. We will take a different approach, we will use field collected data to determine which species should be considered for toxicity testing. Local species and conditions will be considered in all phases of our investigations. This study will investigate several areas of concern regarding atrazine in aquatic systems. It will address the prevalence of atrazine in aquatic systems, examine this prevalence with the known macroinvertebrate fauna, identify aquatic macroinvertebrate fauna that may be indicative of atrazine contamination, and examine what effects atrazine may have on aquatic macroinvertebrates. Because of atrazine's action as an endocrine disrupter in mammalian systems, there is considerable concern with the possible implications exposure may have on human systems. Atrazine is widespread and a common component of surface waters in agricultural areas. Current water testing procedures require extensive chemical analysis. The discovery of a bioindicator for atrazine contamination would be beneficial in documenting possible exposure routes for atrazine to humans.