MOLECULAR BIOLOGY/VIRAL VECTOR CORE

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 建议的分子生物学/病毒载体核心的主要目标是为CEHS研究人员和其他人员提供最先进的支持：1)通过标准分子生物学方法建立高效的、细胞类型特定的和结构性的基因表达克隆；2)使用这些构建物来产生纯化的、高滴度的重组腺相关病毒(AAV)载体。这些服务将提供一种独特的能力，在体外和体内推动特定基因在选定的细胞群体中过度表达或下调。分子生物学/病毒载体核心的总体目的是为研究人员提供一套新的工具，使他们能够实验性地提出关键问题，从而导致高质量的出版物和初步数据的生成，以支持RO1赠款申请。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The primary objective of the proposed molecular biology/viral vector core is to provide state of the art support for CEHS investigators and others to: 1) establish highly efficient, cell type specific and constitutive gene expression clones through standard molecular biology methodologies and; 2) use these constructs to generate purified, high titer recombinant adeno-associated virus (AAV) vectors. These services will provide a unique ability to drive the over expression or knock down of specific genes in selected cell populations both in vitro and in vivo. The overall purpose of the molecular biology/viral vector core is to provide investigators with a set of novel tools that will enable them to experimentally ask critical questions leading to high quality publications and the generation of preliminary data, to support RO1 grant applications.