STRUCTURAL STUDIES ON COMPLEX METAL-CONTAINING PROTEINS

这个子项目是许多利用 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 我们正在使用X-射线衍射方法来解决有关几个含金属蛋白质的生化机制的问题。除了确定新酶的结构外，我们的主要重点是各种酶的机械相关中间体的结构表征，目的是获得有关酶机制的信息。由于这些研究通常涉及结合底物和抑制剂的表征，因此它们与酶相互作用的细节只能通过高分辨率的X射线衍射数据来揭示。目前，这种方法正在应用于含金属的氢化酶和固氮酶，各种磷酸酶，以及参与烯烃和酮的生物转化的酶。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. We are using x-ray diffraction methods to address issues concerning the biochemical mechanisms of several metal-containing proteins. In addition to determining the structures of novel enzymes, our main focus is the structural characterization of mechanistic relevant intermediates of various enzymes with the goal of obtaining information concerning enzyme mechanism. Since these studies are often concerned with the characteriztion of bound substrates and inhibitors, the details of their interaction with the enzymes can only be revealed with high resolution x-ray diffraction data. Currently, this approach is being applied to metal-containing hydrogenases and nitrogenases, various phosphatases, and enzymes involved in the bioconversion of alkenes and ketones.