INTERACTIONS OF DNA POLYMERASE WITH DNA SUBSTRATES

这个子项目是许多利用 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 C到T和CC到TT转换突变是在皮肤癌中观察到的最常见的突变。 提出了脱氨基假说来解决这些突变的产生。 在这个假设中，胞嘧啶或5-甲基胞嘧啶光产物脱氨基形成尿嘧啶或胸腺嘧啶光产物。 光产物中的那些尿嘧啶或胸腺嘧啶部分优先与腺嘌呤形成沃森-克里克碱基对。 经过两个步骤的DNA复制，C到T转换突变的结果。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. C to T and CC to TT transition mutations are the most frequent mutations observed in skin cancer. A deamination hypothesis was proposed to address the generation of those mutations. In this hypothesis, a cytosine or 5-methylcytosine photoproduct deaminates to form a uracil or thymine photoproduct. Those uracil or thymine moieties in the photoproduct form Watson-Crick base-pair preferentially with adenine. After two steps of DNA replication, C to T transition mutation results.