MODELING ORGANELLE TRAFFICKING

这个子项目是许多利用 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 本研究的目的是以鱼类黑色素细胞为实验系统，研究微管依赖性和肌动蛋白丝依赖性转运之间的协调机制。这些大细胞的唯一功能是快速和同步重新分布数千个膜结合的细胞器，色素颗粒，聚集在细胞中心或重新均匀地分散在整个细胞质。 聚集过程中的色素再分布涉及到运输到MT的负端。色素分散结合了最初快速移动到MT加末端和沿着AF的缓慢扩散样移动，这导致色素在细胞质中的均匀分布。因此，色素转运需要不同细胞骨架特异性和极性的多个马达的活动的协调。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The goal of this project is to understand the mechanisms for the coordination between microtubule (MT)-dependent and actin filament (AF) transport using fish melanophores as an experimental system. The only function of these large cells is fast and synchronous redistribution of thousands of membrane-bounded organelles, pigment granules, which aggregate at the cell center or redisperse uniformly throughout the cytoplasm. Pigment redistribution during aggregation involves transport to the minus ends of MTs. Pigment dispersion combines initial rapid movement to the MT plus ends and slow diffision-like movement along the AFs, which results in the homogeneous distribution of pigment in the cytoplasm. Therefore pigment transport requires coordination of the activities of multiple motors of different cytoskeletal specificity and polarity.