MULTIMODAL SPECTROSCOPY PROBE DEVELOPMENT

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 我们正在研究结合反射率和荧光的诊断信息与被称为多峰光谱(MMS)的拉曼光谱获得的诊断信息在诊断动脉粥样硬化和乳腺癌方面的作用。因此，正在开发一种单一的光谱探测器，以用于所有三种模式。组合的探测器将从现有的反射、荧光和拉曼光源中获取激发光，并将光传输到和收集来自相同组织位置的光。组合探头的设计能够传输和收集300-1000 nm光谱范围内的光。基于已在手术室成功使用的在体拉曼探头，组合探头将能够将多个源耦合到其单一激励光纤中，将激励和发射过滤器与专门设计的光学涂层结合在一起，以抑制光纤背景，并能够有效地提取所需的信号。采集的光纤将被分开，并被引导到临床MMS仪器的两个光谱仪/CCD模块进行光谱分析和参数提取。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. We are investigating the utility of combining diagnostic information from reflectance and fluorescence to that obtained by Raman spectroscopy  termed multimodal spectroscopy (MMS) - to diagnose atherosclerosis and breast cancer. As a result, a single spectral probe is being developed to be used with all three modalities. The combined probe would take excitation light from existing reflectance, fluorescence, and Raman sources and deliver to and collect the light from the same tissue location. The combined probe is designed to be capable of delivering and collecting light in the 300-1000 nm spectral range. Based on the in vivo Raman probe that has been successful used in the surgical theatre, the combined probe will enable the coupling of multiple sources into its single excitation fiber, incorporate excitation and emission filters with specially designed optical coatings to suppress fiber background and enable efficient extraction of the desired signal. Collection fibers would be split up and be directed to two spectrograph/CCD modules of the clinical MMS instrument for spectral analysis and parameter extraction.