SYNTHESIS & CHARACTERIZATION OF NEW MACROMOLECULAR CONTRAST AGENTS

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 成像造影剂(或造影剂)是医学成像中用于提高器官/组织图像对比度和显示器官功能的一类诊断药物。由于大分子造影剂的血池特性，它们在表征肿瘤血管的微血管通透性/渗漏方面具有重要的作用。合成了结构清晰、尺寸均一度高、合成灵活性强、成像效果好的新型聚乙二醇型树状大分子造影剂。它们的平均相对分子质量(MW)和相对分子质量分布将通过质谱学获得，这些信息对于研究它们的结构-性质关系以及指导我们的合成优化具有重要的价值。此外，那些由于图像对比度而产生的新的小分子中间体，需要用质谱仪进行结构表征。用基质辅助激光解吸电离飞行时间(MALDI-TOF)质谱仪测定了不同大分子衍生物(分子量在3,000-90,000)的相对分子质量和相对分子质量分布。对于小分子化合物(相对分子质量100-2000)，可采用电子喷雾电离(ESI)质谱仪。质谱学数据将与物理和成像测量数据相关联，将寻找这类新型树枝状造影剂的化学、物理和成像性能之间的关系。质谱学数据对我们合成用于肿瘤成像的新型聚乙二醇树状大分子造影剂是非常重要和有价值的。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Imaging contrast agents (or contrast media) are a category of diagnostic pharmaceuticals for enhancing the image contrast of organs/tissues and for displaying the organ function in medical imaging. Macromolecular contrast agents have found their important role in the characterization of microvascular permeability/leakiness of tumor vessels, due to their blood-pool characteristics. New polyethyleneglycol (PEG)-core dendrimeric contrast agents with well-defined structure, high degree of size homogeneity, synthetic flexibility, and high effectiveness in imaging, have been synthesized. Their average molecular weights (MW) and MW distribution will be obtained from mass spectrometry, which info will be highly valuable for the study of their structure-property relationship and for the guidance of our synthetic optimization. In addition, those new small molecular intermediates, which are attributed to the image contrast, need the structure characterization by mass spectrometry. For different macromolecular derivatives (MW 3,000 - 90,000), matrix-assisted laser desorption ionization time-of-flight (MALDI-TOF) mass spectrometry is used for the measurements of their MW and MW distributions. For small molecular compounds (MW 100 - 2000), electron spray ionization (ESI) mass spectrometry is applicable. Mass spectra data will be correlated with the data from physical and imaging measurements, the relationship among chemical, physical and imaging properties will be sought for this new class of dendrimeric contrast agents. The mass spectra data are highly important and valuable in the synthesis of our new PEG-core dendrimeric contrast agents for cancer imaging.