MICROARRAY

微阵列 诞生于十多年前，基于微阵列的测定已经成为基础， 转化和相关的临床癌症研究。使用该技术进行的测定类型 这使得数以百万计的同时测量，包括基因表达谱，全球 单核苷酸多态性分析、基因启动子活性的ChIP芯片测定和蛋白质组学分析。 分析。基于微阵列的分析对于发现研究是有价值的，帮助研究人员识别和 表征参与致癌作用的信号转导途径，以及癌症的生物标志物 检测、预后和治疗决策。尽管斑点阵列仍然被使用，特别是对于 随着定制阵列和技术的发展，众多阵列制造商已进入市场 使得该技术更具成本效益和可重复性。共享微阵列的使命 资源是为癌症中心的研究人员提供高质量的标准，尖端，定制 微阵列分析，以及关于微阵列的实验设计和培训/教育的咨询 方法.癌症中心微阵列共享资源自1999年9月以来一直在运作， 当它开始作为一个发展共享资源与机构的支持。DC提供部分资金 圣地亚哥CCSG-开始于2001年4月。多年来，微阵列共享资源已发展到 包括三个独立的实验室：（1）VA基因芯片核心设施，位于UC VA租赁的空间 圣地亚哥斯坦大楼;（2）生物医学基因组学微阵列（BIOGEM）核心设施，位于 位于加州大学圣地亚哥分校拉霍亚校区的Leichtag大楼;以及（3）生物标志物核心实验室，位于 摩尔癌症中心大楼这些核心实验室所提供的设施和服务， 选择不重叠，以减轻微阵列仪器的成本，微阵列仪器经常 在这个快速发展的领域需要升级。就以下方面而言，支持这些设施的联合 组织和资金，由摩尔癌症中心，加州大学圣地亚哥分校医学院， 和圣地亚哥退伍军人医学研究基金会。微阵列资源海外委员会 指导设施的运营政策和技术问题。迄今为止，该基金已为 50多名不同癌症中心研究人员的实验室。预计这种使用将继续下去， 随着微阵列检测成本的降低和新的、计划中的微阵列服务的增加， 请上线。

Microarray Borne little more than a decade ago, microarray-based assays have emerged as a critical tool for basic, translational, and correlative clinical cancer research. The types of assays performed with this technology that enables hundreds to millions of simultaneous measurements include gene expression profiling, global analysis of single nucleotide polymorphisms, ChlP-on-chip assays of gene promoter activity, and proteomic analyses. Microarray-based assays are valuable for discovery research, helping investigators identify and characterize signal transduction pathways involved in carcinogenesis, as well as, biomarkers for cancer detection, prognosis, and treatment decision-making. Although spotted arrays are still used, especially for custom arrays and technology development, numerous array manufacturers have entered the marketplace making the technology much more cost-effective and reproducible. The mission of the Microarray Shared Resource is to provide Cancer Center investigators with high quality standard, cutting-edge, and custom microarray assays, as well as, consultation on experimental design and training/education about microarray methods. The Cancer Center Microarray Shared Resource has been in operation since September, 1999, when it began as a developmental shared resource with institutional support. Partial funding from the DC San Diego CCSG-started in April, 2001. Over the years the Microarray Shared Resource has grown to include three separate laboratories: (1) the VA GeneChip Core facility, located in VA-leased space in the UC San Diego Stein Building; (2) the Biomedical Genomics Microarray (BIOGEM) Core facility, located in the Leichtag Building on the UC San Diego La Jolla campus; and (3) the Biomarker Core laboratory, located in the Moores Cancer Center Building. The facilities and services provided these Core laboratories have been selected to be non-overlapping in order to mitigate the costs of microarray instrumentation, which frequently need upgrading for this rapidly evolving field. The confederation of these facilities is supported, in terms of organization and funding, by the administration of the Moores Cancer Center, the UCSD School of Medicine, and the San Diego Veterans Medical Research Foundation. The Microarray Resource Oversite Committee directs the operational policies and technology issues for the Facility. To date, the Facility has served the laboratories of more than 50 different Cancer Center investigators. It is anticipated that this use will continue to expand as costs for microarray assays decrease and new, planned microarray services in the Facility come online.