FLOW CYTROMETRY

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Flow Cytometry The Flow Cytometry Shared Resource has been an integral component of the UCSD Cancer Center since 1990. It exists to provide peer-review, funded investigators from all Divisions of the Cancer Center with ready access to high-speed analysis and sorting of dissociated cell populations from clinical samples, animal experiments and cell culture studies. The services available from this Resource are as follows: Analytical Flow Cytometry: Utilizes a FACSCalibur¿ flow cytometer and a newly purchased FACSAria¿ flow cytometer. The FACSCalibur¿ is a bench-top flow cytometer that can be used for analysis of up to fourcolors of fluorescence. The FACSAria¿ is a three-laser flow cytometer for analyses capable of up to eleven colors of fluorescence, along with forward and side-anglejight scatter. The FACSAria¿ utilizes FACSDiva¿ software, which has many advanced features, such as biexponential scaling, adaptive gating, and automated fluorescence spectral compensation. The FACSCalibur¿ flow cytometer computer system uses CELLQuest¿ software for list-mode data recording and analysis. These services allow investigators to analyze cell populations for cell cycle status, surface and cytoplasmic antigen phenotype, apoptosis, or expression of a transgene (e.g. following transduction with green fluorescent protein [GFP]).