TRANSGENIC MOUSE

转基因小鼠 许多生物医学科学界人士强烈认为，癌症研究的进展，以及癌症的地位 加州大学圣迭戈分校癌症中心在未来十年的生物医学界，将取决于能力 用小鼠作为实验模型来研究基础和临床相关的问题 癌症研究。通过基因操作建立新的小鼠癌症临床前模型 NCI特别指出，这是他们希望利用的四个非凡机会之一。转基因小鼠 携带新的或新的基因是通过将DMA显微注射到受精卵的原核中而产生的 缺乏特定感兴趣基因的“敲除”小鼠是通过胚胎中的同源重组而产生的 干细胞，然后注射到囊胚中，创造嵌合体小鼠，以繁殖到纯合子。这个 人类和小鼠基因组中大多数序列的高度保守使得使用 小鼠基因操作技术极大地建立人类癌症发病机制模型 很有吸引力。这些方法在癌症研究中非常强大，特别是在分析 癌基因、转移、细胞周期控制、肿瘤抑制基因以及在癌症模型制作中的作用 用于开发新治疗方案的系统，以及用于药物测试和肿瘤成像的方法。这个 这个共享资源的使命是提供基因操作的高科技方面 胚胎和胚胎干细胞为我们的中心成员提供服务，使他们能够创造 他们需要基因操控的小鼠模型。这是一个突出的例子，说明了 技术、训练有素的专职人员和昂贵的设备可以供研究人员使用 他们不能合理地期望在个人的基础上开发或获得它们。这款产品的可用性 资源使我们的研究人员能够通过一系列复杂的 基因技术。

Transgenic Mouse Many in the biomedical sciences strongly believe that the progress of cancer research, and the position of the UCSD Cancer Center in the biomedical community during the next decade, will depend upon the ability to use the mouse as an experimental model to investigate both basic and clinically relevant questions in cancer research. The creation of new pre-clinical models of cancer in mice by genetic manipulation is specifically cited by the NCI as one of four extraordinary opportunities they wish to exploit. Transgenic mice carrying new or novel genes are created by microinjection of DMA into the pronuclei of fertilized eggs and "knock-out" mice lacking specific genes of interest are created by homologous recombination in embryonic stem cells followed by injection into blastocysts to create chimeric mice for breeding to homozygosity. The high degree of conservation of most sequences in genomes of humans and mice makes the idea of using mouse genetic manipulation technology to create models of human cancer pathogenesis extremely attractive. These approaches are remarkably powerful in cancer research particularly in the analysis of oncogenes, metastasis, cell-cycle control, tumor suppressor genes, and in the crafting of cancer model systems for developing new treatment regimens, and methods for drug testing and tumor imaging. The mission of this Shared Resource is to provide the highly technical aspects of manipulation of genes in embryos and embryonic stem cells as a service to our Center members, allowing them to create the genetically manipulated mouse models they need. This is an outstanding example of how specialized techniques, highly trained dedicated personnel, and expensive equipment can be accessed by researchers who could not reasonably expect to develop or obtain them on an individual basis. The availability of this Resource enables our researchers to conduct versatile, cutting-edge research with a battery of sophisticated genetic techniques.