Stimulation of tooth repair and regeneration by interaction with dental meterials

描述（由申请人提供）：本申请的总体目标是为候选人（一名在牙科材料研究方面具有广泛背景的高级研究员）提供用于研究因牙科疾病造成的损伤或损害及其与牙科材料的后续相互作用而导致的活牙组织、牙髓和牙本质再生的技术经验。再生医学的进步正在为恢复性牙科的新时代铺平道路，这项培训将使候选人成为为这一领域做出贡献的关键人物。这方面的经验，包括具体的研究项目，是在支持NIDCR的目标，这是鼓励研究的修复和再生的口面组织，以确定常用的牙科材料的安全性，并提供足够的和训练有素的研究队伍。为了完成这个总体目标，候选人在赞助人托尼·史密斯博士的建议下设计了一系列实验。伯明翰），这一领域的专家。牙本质粘合剂和玻璃离聚物清洁剂（含和不含生物活性玻璃添加剂）将用作试验材料。这项高级研究金申请的研究计划有三个具体目标。第一个目的是用系统性更高强度（较低pH）的酸处理人类牙本质，这代表了当前“自取”牙科粘合剂以及自蚀刻粘合剂和常规玻璃离聚物的商业版本的差异，并鉴定和定量释放的生物活性分子，如生长因子。第二个目的是使用细胞培养和基因表达来检查由各种材料从牙本质释放的生物活性分子在其刺激牙本质再生的能力方面的活力。第三个目的是利用伯明翰大学开发的牙片器官培养模型，研究暴露于特定牙科材料的牙本质的细胞死亡程度和愈合过程;并确定通过将钙和磷酸盐释放材料，二氧化硅基生物活性玻璃，申请人目前正在研究将其用于牙科粘合剂和清洁剂。公共卫生相关性：这项工作与公共卫生有关，因为它调查了牙齿组织对用于修复牙齿和自我修复和再生的材料的反应方式。这项工作还试图通过释放牙齿修复或再生过程所需的自身分子来确定可能有助于愈合过程的牙科材料。

DESCRIPTION (provided by applicant): The overall goal of this application is to provide the candidate, a senior investigator with an extensive background in dental materials research, with experience in the techniques used to study the regeneration of living dental tissues, pulp and dentin, as a result of injury or insult from dental disease and their subsequent interactions with dental materials. Advances in regenerative medicine are paving the way for a new era in restorative dentistry and this training will position the candidate to be a key player in contributing to this field. This experience, including the specific research project, are in support of the goals of the NIDCR which is to encourage research on the repair and regeneration of orofacial tissues, to determine the safety of commonly used dental materials, and to provide an adequate and well-trained research workforce. To accomplish the overall goal, a series of experiments have been designed by the candidate, with the advice of the sponsor, Dr. Tony Smith (U. Birmingham), an expert in this field. Dentin adhesives and glass ionomer restoratives, with and without a bioactive glass additive, will serve as the test materials. The research plan for this senior fellowship application has three specific aims. The first aim is to treat human dentin with acid of systematically greater strength (lower pH), representing differences in current "selfetching" dental adhesives, as well as commercial versions of self-etching adhesives and conventional glass ionomer, and to identify and quantitate the bioactive molecules, such as growth factors, that are released. The second aim is to use cell culture and gene expression to examine the viability of the bioactive molecules released from dentin by the various materials in terms of their ability to stimulate dentin regeneration. The third aim is to use the tooth slice organ culture model developed at the University of Birmingham to study the extent of cell death and the healing process of dentin exposed to specific dental materials; and to determine if the healing process can be enhanced by incorporating into the self-etching adhesive and glass ionomer a calcium and phosphate releasing material, a silica-based bioactive glass, that is currently being studied by the applicant for use in dental adhesives and restoratives. Public Health Relevance: This work is relevant to public health in that it investigates the manner in which tooth tissues respond to materials used to restore teeth and repair and regenerate themselves. This work also seeks to identify dental materials that may aid in the healing process by releasing the tooth's own molecules needed for the repair or regeneration process.