IMPRINTED GENES IN NORMAL AND ABNORMAL PLACENTAL/FETAL FUNCTION

胎儿宫内生长迟缓（IUGR）是婴儿死亡和发病的重要原因，影响超过100万婴儿的健康。 8%的孕妇。IUGR相关并发症包括宫内死亡、产时胎儿窘迫， 围产期窒息这是除了产前的困难和增加成人的倾向， 冠心病、高血压和糖尿病等疾病。虽然IUGR的原因是多种多样的， 在很大比例的情况下，它与胎盘有关！功能障碍胎盘是一个复杂的 除作为活性物质外，还执行包括营养物和废物转移在内的许多功能的器官 内分泌器官印记基因在胎盘中起着重要作用！，胎儿和神经发育。筛余的大 目前已知的75个印迹基因中，21个涉及胎盘特异性印迹模式， 表达异常时胎盘功能的破坏，表明它们在胎盘功能中的关键作用。 为了阐明印迹基因在IUGR中的作用，我们建议对IUGR中的印迹基因进行详细的分析。 已知印迹基因在正常和IUGR胎盘中的表达。此外，我们还开发了一种模型， 用于鉴定参与胎盘运输功能的新的印记基因。这些小说的表达方式 基因也将在正常和IUGR胎盘中进行研究。我们建议比较基因表达谱 单亲和双亲组织，以及IUGR和正常胎盘。所产生的信息将被汇编， 和选择用于进一步分析的候选基因。基因表达水平和SNPs关联分析将 在IUGR和正常妊娠的胎盘中进行

Intrauterine growth retardation (IUGR) is a significant cause of infant mortality and morbidity, affecting over 8% of all pregnancies. lUGR-related complications include intrauterine death, intrapartum fetal distress, and perinatal asphyxia. This is in addition to both prenatal difficulties and increased adult predisposition to ailments such as coronary heart diseases, hypertension and diabetes. While the causes of IUGR are varied, in a significant proportion of cases it is associated with placenta! dysfunction. The placenta is a complex organ that performs many functions including nutrient and waste transfer, in addition to serving as an active endocrine organ. Imprinted genes play a major role in placenta!, fetal and neural development. Of the over 75 imprinted genes presently known, 21 are implicated in either placental-specific imprinting patterns or disruption of placental function when expressed abnormally, indicating their critical role in placental function. In order to elucidate the role of imprinted genes in IUGR we propose to undertake a detailed analysis of known imprinted gene expression in normal and IUGR placentas. In addition, we have developed a model for identifying novel imprinted genes involved in placental transport functions. The expression of these novel genes will also be studied in normal and IUGR placentas. We propose to compare gene expression profiles of uni- and bi-parental tissues, and IUGR and normal placentas. The information generated will be compiled, and candidate genes selected for further analysis. Gene expression level and SNPs association analysis will be carried out in placentas from IUGR and normal pregnancies