HIGH MASS-TO-CHARGE FOURIER TRANSFORM MASS SPECTROMETRY

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 本研究的目的是继续努力了解离子运动的修改FTMS陷阱，构建陷阱的理论考虑为指导，耦合的陷阱与高压事件（称为射频模式事件）的离子和MS/MS的碰撞聚焦，并测试的有效性的高压事件和电补偿细胞研究MALDI产生的高质量离子。 该核心研究项目的目标是通过改变陷阱的电学性质而不是通过增加磁场强度来改善质量电荷比大于几千的生物分子离子的检测和MS/MS性能。 我们建议，仪器将不仅是中型蛋白质的兴趣，但也为肽在蛋白质组学应用。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The purposes of the research are to continue efforts to understand ion motion in modified FTMS traps, to construct traps guided by the theoretical considerations, to couple the trap with a high-pressure event (called the rf-only mode event) for collisional focusing of ions and MS/MS, and to test the effectiveness of the high-pressure event and an electrically compensated cell to study high-mass ions generated by MALDI. The goal of this core research project is to improve the detection and MS/MS performance for biomolecule ions with mass-to-charge ratios greater than a few thousand by making changes to the electrical properties of the trap rather than by increasing the magnetic field strength. We suggest that the instrumentation will not only be of interest for mid-sized proteins but also for peptides in proteomics applications.