Analytical Core

分析核心实验室将为项目内的个别项目提供生化和基因分型分析服务，以及组织学和免疫组织化学分析。生化检测包括ET-1、MCP-1、sICAM、IL-6、cGMP、硝酸盐/亚硝酸盐、微量白蛋白、组织总蛋白、醛固酮、尿白蛋白、对氨基马尿酸、胰岛素、红细胞压积、电解质肌酐、8-异前列腺素、硫代巴比托酸反应物、超氧化物、尿液、血浆和/或大鼠、小鼠和人类组织提取物的测量。技术将包括放射免疫测定、ELISA、离子选择电极、酶测定和结合测定。对由Core c维持的转基因大鼠和小鼠进行基因分型分析。组织和免疫组织化学分析将为项目提供苏木精和伊红（H&E）、周期性酸希夫（PAS）、天狼星红、马森蓝三色和特定蛋白质标记的特异性免疫组织化学染色分析。该核心还将根据个别研究者的需要，为开发额外的检测和/或组织学和免疫组织学分析提供资源。核心将通过验证程序确保检测和组织学质量控制。

The Analytical Core laboratory will provide biochemical and genotyping assay services as well as histology and immunohistochemical analyses for the individual projects within the Program Project. Biochemical assays that will be performed include ET-1, MCP-1, sICAM, IL-6, cGMP, nitrate/nitrite, microalbumin, tissue total protein, aldosterone, urinary albumin, para-aminohippuric acid, inulin, hematocrit, electrolytes creatinine, 8-isoprostane, thiobarbituric acid reactants, superoxide, measurements in urine, plasma, and/or tissue extracts from rats, mice, and humans. Techniques will include radioimmunoassay, ELISA, ion selective electrodes, and enzymatic assays, and binding assays. Genotyping assays will also be accomplished for the transgenic rats and mice that will be maintained by Core C. Histology and immunohistochemical analyses will provide hematoxylin & eosin (H&E), periodic acid-Schiff (PAS), Sirius red, Masson's blue trichrome, and specific immunohistochemical staining analyses for specific protein markers for the Program Project. The core will also provide resources for development of additional assays and/or histological and immunohistological analyses as needed by individual investigators. The core will assure assay and histology quality control through validation procedures.