CORE D CELL CULTURE AND VIRAL VECTOR CORE

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 该核心成立于2004年，旨在满足对高质量细胞和重组病毒日益增长的需求，以支持COBRE在血管生物学项目中的应用。 核心负责：（i）人和鼠内皮和血管平滑肌细胞的获取、制备和储存;（ii）来源于新品系小鼠的细胞的永生化，和（iii）制备编码用于基因转移实验的感兴趣的信号分子的高滴度腺病毒、逆转录病毒和慢病毒储液。 预计使用来自共同来源的高质量细胞和病毒试剂将导致合作实验室之间更有效，更具成本效益和更可重复的实验。 与所有其他核心一样，已经实施了服务收费，因此当COBRE资金不再可用时，核心可以自给自足。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. This Core was established in 2004 to meet the increasing demand for high quality cells and recombinant viruses in support of COBRE in Vascular Biology projects. The Core is responsible for: (i) the procurement, preparation, and storage of human and murine endothelial and vascular smooth muscle cells; (ii) immortalization of cells derived from novel strains of mice, and (iii) preparation of high titer adenoviral, retroviral, and lentiviral stocks encoding the signaling molecules of interest for gene transfer experiments. It is anticipated that the use of high quality cells and viral reagents from a common source will result in more efficient, cost-effective, and more reproducible experiments between collaborating laboratories. As with all other cores, fees-for-service have been implemented so the Core may become self-sufficient when COBRE funds are no longer available.