PROJECT 1: P-MESOGENIN1 IN MESODERMAL DIFFERENTIATION

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 我们的研究集中在胚胎干细胞中中胚层分化的分子、细胞和遗传调控以及发育过程中。我们已经研究了bHLH转录因子pMesgenin1，它是正常的近轴中胚层发育所必需的。为了了解pMesgenin1‘S的作用机制，我们对pMesgenin1缺失和野生型胚胎进行了转录组分析。在pMesgenin1缺失的样本中，一个表达增加的基因编码了一种新的分泌蛋白Cristin1(富含半胱氨酸的单一TSR结构域包含蛋白1，也称为R-Spin3)。在搜索序列数据库中，我们在人和小鼠基因组中发现了三个Cristin1同源物(Cristin 2，3和4)。我们目前的研究工作主要集中在利用分子、细胞生物学和遗传学方法在组织培养和小鼠模型中确定R-响应蛋白家族的体内作用和信号机制。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Our research focuses on the molecular, cellular, and genetic regulation of mesoderm differentiation in embryonic stem (ES) cells, and during development. We have studied the bHLH transcription factor, pMesogenin1, which is required for proper paraxial mesoderm development. Towards understanding pMesogenin1's action mechanisms, we performed transcriptome analyses in pMesogenin1 null and wild type embryos. One gene whose expression was increased in pMesogenin1-deficient samples encodes a novel secreted protein, Cristin1 (cysteine-rich and single TSR domain containing protein 1, also known as R-spondin3). In searching sequence databases, we identified three Cristin1 homologues in the human and mouse genome (Cristin 2, 3, and 4). Our current research efforts focus primarily on determining the in vivo roles and signaling mechanisms of the R-spondin family utilizing molecular, cell biological and genetic approaches in tissue culture and mouse models.