PROJECT 1: P-MESOGENIN1 IN MESODERMAL DIFFERENTIATION

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 我们的研究重点是胚胎干细胞（ES）中胚层分化的分子，细胞和遗传调控，并在发展过程中。我们已经研究了bHLH转录因子，pMesogenin 1，这是所需的适当近轴中胚层的发展。 为了了解pMesogenin 1的作用机制，我们在pMesogenin 1 null和野生型胚胎中进行了转录组分析。在pMesogenin 1缺陷样品中表达增加的一个基因编码一种新的分泌蛋白，Cristin 1（富含半胱氨酸且含有单一TSR结构域的蛋白1，也称为R-spondin 3）。 在搜索序列数据库中，我们在人类和小鼠基因组中确定了三个Cristin 1同源物（Cristin 2，3和4）。我们目前的研究工作主要集中在确定在体内的作用和信号转导机制的R-spondin家族利用分子，细胞生物学和遗传学方法在组织培养和小鼠模型。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Our research focuses on the molecular, cellular, and genetic regulation of mesoderm differentiation in embryonic stem (ES) cells, and during development. We have studied the bHLH transcription factor, pMesogenin1, which is required for proper paraxial mesoderm development. Towards understanding pMesogenin1's action mechanisms, we performed transcriptome analyses in pMesogenin1 null and wild type embryos. One gene whose expression was increased in pMesogenin1-deficient samples encodes a novel secreted protein, Cristin1 (cysteine-rich and single TSR domain containing protein 1, also known as R-spondin3). In searching sequence databases, we identified three Cristin1 homologues in the human and mouse genome (Cristin 2, 3, and 4). Our current research efforts focus primarily on determining the in vivo roles and signaling mechanisms of the R-spondin family utilizing molecular, cell biological and genetic approaches in tissue culture and mouse models.