PROJECT 1: P-MESOGENIN1 IN MESODERMAL DIFFERENTIATION

项目 1：P-mesogenin1 在中胚层分化中的作用

• 批准号: 7720700
• 负责人: JEONG YOON
• 金额: $ 22.27万
• 依托单位: MAINEHEALTH
• 依托单位国家: 美国
• 财政年份: 2008
• 资助国家: 美国
• 起止时间: 2008-09-04 至 2009-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7720700
• 关键词: Biological; Cells; Computer Retrieval of Information on Scientific Projects Database; Cysteine; Databases; Development; Embryo; Family; Funding; Gene Expression Profile; Genes; Genetic; Grant; Homologous Gene; Human; Institution; Mesoderm; Molecular; Paraxial Mesoderm; Proteins; Regulation; Research; Research Personnel; Resources; Role; Sampling; Signal Transduction; Source; United States National Institutes of Health; embryonic stem cell; in vivo; mouse genome; mouse model; novel; tissue culture; transcription factor

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Our research focuses on the molecular, cellular, and genetic regulation of mesoderm differentiation in embryonic stem (ES) cells, and during development. We have studied the bHLH transcription factor, pMesogenin1, which is required for proper paraxial mesoderm development. Towards understanding pMesogenin1's action mechanisms, we performed transcriptome analyses in pMesogenin1 null and wild type embryos. One gene whose expression was increased in pMesogenin1-deficient samples encodes a novel secreted protein, Cristin1 (cysteine-rich and single TSR domain containing protein 1, also known as R-spondin3). In searching sequence databases, we identified three Cristin1 homologues in the human and mouse genome (Cristin 2, 3, and 4). Our current research efforts focus primarily on determining the in vivo roles and signaling mechanisms of the R-spondin family utilizing molecular, cell biological and genetic approaches in tissue culture and mouse models.

该子项目是利用该技术的众多研究子项目之一 资源由 NIH/NCRR 资助的中心拨款提供。子项目和 研究者 (PI) 可能已从 NIH 的另一个来源获得主要资金， 因此可以在其他 CRISP 条目中表示。列出的机构是 对于中心来说，它不一定是研究者的机构。 我们的研究重点是胚胎干 (ES) 细胞和发育过程中中胚层分化的分子、细胞和遗传调控。我们研究了 bHLH 转录因子 pMesogenin1，它是轴旁中胚层正常发育所必需的。 为了了解 pMesogenin1 的作用机制，我们在 pMesogenin1 缺失和野生型胚胎中进行了转录组分析。在 pMesogenin1 缺陷样品中表达增加的一个基因编码一种新型分泌蛋白 Cristin1（富含半胱氨酸且含有单个 TSR 结构域的蛋白 1，也称为 R-spondin3）。 在搜索序列数据库时，我们在人类和小鼠基因组中鉴定了三个 Cristin1 同源物（Cristin 2、3 和 4）。我们目前的研究工作主要集中在利用组织培养和小鼠模型中的分子、细胞生物学和遗传学方法确定 R-spondin 家族的体内作用和信号传导机制。