CORE--Gene Microarray

基因微阵列共享资源(GMSR)是一个全方位服务的DMA微阵列设施，为OHSU癌症研究所(CL)的研究人员提供合理成本的高质量表达谱服务。核心设施有一名全职博士主任、一名实验室经理、两名研究技术人员和一名信息学家。服务包括：1)实验设计和样品制备咨询；2)基因芯片寡核苷酸阵列表达谱分析；3)数据管理和存储；4)使用配备高性能PC和微阵列分析软件的信息学实验室；5)支持微阵列数据分析；以及6)关于微阵列技术、数据格式和数据分析软件的教育和培训。数据管理系统通过信息学和生物统计共享资源进行维护，高级数据分析也可通过 中芯修女。我们已经培养了一大批专业人员和研究人员，他们具有基本的能力、过去在微阵列技术方面的经验和复杂性，以确保适当的实验设计和解释。我们完善的基础设施和质量控制程序支持将快速、可重复和准确的微阵列数据提供给氯仿研究人员。在过去的三年里，GMSR为CI成员处理了50多个基于Affymetrix阵列的项目，吞吐量超过600个阵列。自五年前发展以来，CL成员也可以通过这种共享资源获得斑点微阵列服务。

The Gene Microarray Shared Resource (GMSR) is a full service DMA microarray facility providing OHSU Cancer Institute (Cl) investigators with high-quality expression profiling services at reasonable cost. The core facility has a full-time PhD director, a laboratory manager, two research technicians and an informaticist. Services include: 1) consultation on experiment design and sample preparation; 2) expression profiling on GeneChip oligonucleotide arrays; 3) data management and storage; 4) access to an informatics laboratory equipped with high-performance PCs and microarray analysis software; 5) support for microarray data analysis; and 6) education and training on microarray technology, data formats, and data analysis software. Data management systems are maintained through the Informatics and the Biostatistics Shared Resources, and advanced data analysis is also available through this sister Cl core. We have developed a critical mass of professionals and investigators with essential capabilities, past experience in microarray technology, and sophistication to assure appropriate design and interpretation of experiments. Our well-established infrastructure and quality-controlled procedures support the delivery of rapid, reproducible and accurate microarray data to Cl investigators. In the past three years, the GMSR has processed over 50 Affymetrix array-based projects for Cl members with a throughput of over 600 arrays. Cl members have also had access to spotted microarray services through this shared resource since its development five years ago.